Vasquez-Valverde Luisa Fernanda, Marek Paul E
Virginia Tech, Department of Entomology, 170 Drillfield Drive, Blacksburg, Virginia 24061, USA Virginia Tech, Department of Entomology Blacksburg United States of America.
Zookeys. 2022 Jun 20;1106:141-163. doi: 10.3897/zookeys.1106.81386. eCollection 2022.
The millipede genus Chamberlin, 1949 is a monospecific taxon, with the type species (Bollman, 1889). The last revision of the genus was made by Hoffman (1960) where he established three subspecies. Here we used molecular phylogenetics to assess the genus and evaluate whether it is a monophyletic group, and if the subspecies are each monophyletic. We included material from literature records and three natural history collections. Newly collected samples were obtained through a citizen science project. Morphological characters underlying subspecies groups-the shape of the paranota, body size, and coloration-were evaluated. A molecular phylogeny of the genus was estimated based on DNA sequences for seven gene loci, and a species delimitation analysis was used to evaluate the status of the subspecies. The documented geographical range of in the United States was expanded to include a newly reported state record (Virginia) and about 160 new localities compared to the previously known range. Morphological characters, which included the shape of the paranota and body size that had been historically used to establish subspecies, showed clinal variation with a direct relationship with geographical distribution and elevation, but not with phylogeny. Coloration was highly variable and did not accord with geography or phylogeny. The phylogeny recovered as a monophyletic lineage, and the species delimitation test supported the existence of a single species. The subspecies (Chamberlin, 1939) and Hoffman, 1960 have been synonymized with . The molecular and morphological evidence showed that is a monospecific genus with the sole species, , being geographically widespread and highly variable in its morphology.
1949年的千足虫属是一个单种分类单元，模式种为（博尔曼，1889年）。该属的上一次修订由霍夫曼（1960年）完成，他在此处确立了三个亚种。在此，我们运用分子系统发育学来评估该属，并判断它是否为一个单系类群，以及各亚种是否分别为单系类群。我们纳入了文献记录中的材料以及三个自然历史收藏。通过一个公民科学项目获取了新采集的样本。对构成亚种组的形态特征——侧缘突的形状、身体大小和颜色——进行了评估。基于七个基因位点的DNA序列估计了该属的分子系统发育，并使用物种界定分析来评估亚种的地位。在美国，该物种已记录的地理分布范围得到扩展，新增了一个州的记录（弗吉尼亚州），与之前已知的范围相比增加了约160个新地点。形态特征，包括历史上用于确立亚种的侧缘突形状和身体大小，呈现出渐变变异，与地理分布和海拔直接相关，但与系统发育无关。颜色变化很大，与地理或系统发育不相符。系统发育分析将其恢复为一个单系谱系，物种界定测试支持单一物种的存在。亚种（钱伯林，1939年）和霍夫曼，1960年已被同义化为。分子和形态学证据表明，这是一个单种属，唯一的物种在地理上广泛分布，形态变化很大。
