Mahmoud Abdel Rahman Hassan, Abdelkawi Salwa Ahmed, Ghoneim Dina Fouad, Hassan Aziza Ahmed, Morsy Mona Ebrahim
Department of Medical Application of Laser, Ophthalmic Unit, National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Department of Vision Science, Biophysics and Laser Science Unit, Research Institute of Ophthalmology, Giza, Egypt.
Oman J Ophthalmol. 2022 Nov 2;15(3):347-352. doi: 10.4103/ojo.ojo_251_21. eCollection 2022 Sep-Dec.
This study aimed to assess the effect of low-level laser therapy (LLLT) and chia seeds on the mitigation of photoreceptors abnormalities in experimental diabetic retinopathy (DR).
A total of 65 female Wistar rats, 5 rats were served as a control group and 60 rats were injected intraperitoneally with one dose of 55 mg/kg of streptozotocin (STZ) to induce DR after 6-8 weeks. The rats were divided into ( = 20 rats each): (a) DR group: did not receive any treatment, (b) DR+ LLLT group was exposed to 670-nm LLLT for 6 weeks (two sessions/week), and (c) DR+ LLLT+ chia seed group, in which rats were exposed to LLLT and administrated with 250 mg/kg/day of chia seeds flour for 2 weeks before STZ injection and continued to the end of the experiment. Blood glucose (BG) levels and retinal histological examination were employed after 1, 2, 4, and 6 weeks.
The BG level in the DR group and the treated groups were significantly higher ( < 0.001) than in the control group after the four-time periods. Chia seeds group exhibited BG levels less than the DR and the DR+ LLLT groups after 6 weeks ( < 0.01). LLLT improved the degeneration of the photoreceptors after 6 weeks of treatment, while LLLT+ chia seeds supplementation showed early photoreceptors improvement after 2 weeks.
The early improvement in the photoreceptors after LLLT+ chia seed may be attributed to the potent antioxidant properties of chia seeds. Therefore, the combination between LLLT and chia seeds should be employed to protect the retinal photoreceptors against DR.
本研究旨在评估低强度激光疗法（LLLT）和奇亚籽对实验性糖尿病视网膜病变（DR）中光感受器异常的缓解作用。
总共65只雌性Wistar大鼠，5只作为对照组，60只大鼠腹腔注射一剂55mg/kg链脲佐菌素（STZ）以诱导糖尿病视网膜病变，6 - 8周后进行实验。大鼠被分为（每组 = 20只大鼠）：（a）糖尿病视网膜病变组：未接受任何治疗；（b）糖尿病视网膜病变 + LLLT组：接受670nm的LLLT照射6周（每周两次）；（c）糖尿病视网膜病变 + LLLT + 奇亚籽组，在注射STZ前2周，大鼠接受LLLT照射并给予250mg/kg/天的奇亚籽粉，持续至实验结束。在1、2、4和6周后检测血糖（BG）水平并进行视网膜组织学检查。
在四个时间段后，糖尿病视网膜病变组和治疗组的BG水平均显著高于对照组（P < 0.001）。6周后，奇亚籽组的BG水平低于糖尿病视网膜病变组和糖尿病视网膜病变 + LLLT组（P < 0.01）。治疗6周后，LLLT改善了光感受器的退化，而LLLT + 奇亚籽补充剂在2周后显示光感受器早期改善。
LLLT + 奇亚籽后光感受器的早期改善可能归因于奇亚籽强大的抗氧化特性。因此，应采用LLLT和奇亚籽的组合来保护视网膜光感受器免受糖尿病视网膜病变的影响。
