Takeuchi Shunsuke, Fukuba Shunsuke, Okada Munehiro, Nohara Kohei, Sato Ryo, Yamaki Daichi, Matsuura Takanori, Hoshi Shu, Aoki Kazuhiro, Iwata Takanori
Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Regen Ther. 2023 Jan 24;22:128-135. doi: 10.1016/j.reth.2023.01.002. eCollection 2023 Mar.
This study aimed to histologically compare periodontal regeneration of one-wall intrabony defects treated with open flap debridement, β-tricalcium phosphate (β-TCP), and carbonate apatite (COAp) in dogs.
The mandibular third premolars of four beagle dogs were extracted. Twelve weeks after the extraction, a one-wall bone defect of 4 mm × 5 mm (mesio-distal width × depth) was created on the distal side of the mandibular second premolar and mesial side of the fourth premolar. Each defect was randomly allocated to open flap debridement (control group), periodontal regeneration utilizing β-TCP, or COAp. Eight weeks after the surgery, histologic and histometric analyses were performed.
No ankylosis, infection, or acute inflammation was observed at any of the experimental sites. Newly formed bone and cementum were observed in all experimental groups. The mineral apposition rate of the alveolar bone crest was higher in the COAp group than in the control and β-TCP groups. The ratio of the new bone area was significantly higher in the COAp group than in the control group ( < 0.05). The bone contact percentage of the residual granules was significantly higher in the COAp group than in the β-TCP group ( < 0.05).
Although this study has limitations, the findings revealed the safety and efficacy of COAp for periodontal regeneration in one-wall intrabony defects in dogs, and COAp has a better ability to integrate with bone than β-TCP.
本研究旨在通过组织学方法比较在犬类中，采用开放瓣清创术、β-磷酸三钙（β-TCP）和碳酸磷灰石（COAp）治疗单壁骨内缺损后的牙周组织再生情况。
拔除四只比格犬的下颌第三前磨牙。拔牙12周后，在下颌第二前磨牙远中侧和第四前磨牙近中侧制造一个4毫米×5毫米（近远中宽度×深度）的单壁骨缺损。每个缺损随机分配至开放瓣清创术（对照组）、使用β-TCP的牙周组织再生治疗或使用COAp的治疗。术后8周，进行组织学和组织计量学分析。
在任何实验部位均未观察到骨粘连、感染或急性炎症。在所有实验组中均观察到新形成的骨和牙骨质。COAp组牙槽嵴的矿物质沉积率高于对照组和β-TCP组。COAp组的新骨面积比例显著高于对照组（<0.05）。COAp组残余颗粒的骨接触百分比显著高于β-TCP组（<0.05）。
尽管本研究存在局限性，但研究结果揭示了COAp在犬类单壁骨内缺损牙周组织再生中的安全性和有效性，且COAp与骨整合的能力优于β-TCP。
