VIB Center for Inflammation Research, 9052 Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
Nucleic Acids Res. 2023 Feb 28;51(4):1652-1661. doi: 10.1093/nar/gkad064.
The Mousepost 1.0 online search tool, launched in 2017, allowed to search for variations in all protein-coding gene sequences of 36 sequenced mouse inbred strains, compared to the reference strain C57BL/6J, which could be linked to strain-specific phenotypes and modifier effects. Because recently these genome sequences have been significantly updated and sequences of 16 extra strains added by the Mouse Genomes Project, a profound update, correction and expansion of the Mousepost 1.0 database has been performed and is reported here. Moreover, we have added a new class of protein disturbing sequence polymorphisms (besides stop codon losses, stop codon gains, small insertions and deletions, and missense mutations), namely start codon mutations. The current version, Mousepost 2.0 (https://mousepost.be), therefore is a significantly updated and invaluable tool available to the community and is described here and foreseen by multiple examples.
Mousepost 1.0 在线搜索工具于 2017 年推出，允许搜索 36 种已测序近交系小鼠所有编码蛋白基因序列的变异，与参考株 C57BL/6J 相比，可与株特异性表型和修饰效应相关联。因为最近这些基因组序列已经显著更新，并且 Mouse Genomes Project 又增加了 16 个额外的品系序列，所以对 Mousepost 1.0 数据库进行了深度更新、修正和扩展，并在此报告。此外，我们还添加了一类新的蛋白质干扰序列多态性（除了终止密码子缺失、终止密码子获得、小插入和缺失以及错义突变），即起始密码子突变。目前的版本 Mousepost 2.0（https://mousepost.be）是社区可用的一个经过显著更新和非常有价值的工具，在此进行了描述，并通过多个示例进行了预见。
