Li Yiming, Huang Ziqing, Huang Xiaoqiong, Xu Ruogu, He Yi, Deng Feilong, Chen Guanhui
Guangdong Provincial Key Laboratory of Stomatology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Department of Stomatology, the Seventh Affiliated Hospital, Sun Yat-sen University, Guangdong, China.
J Biomed Mater Res B Appl Biomater. 2023 Jun;111(6):1309-1317. doi: 10.1002/jbm.b.35234. Epub 2023 Feb 10.
Guided bone regeneration (GBR) is a frequently used technique for patients with insufficient alveolar bone. The discovery of bone substitutes that can enhance osteogenesis is critical for GBR. Graphdiyne (GDY), a newly discovered carbon-based nanomaterial, has been recognized as the most stable allotrope of acetylene carbon and is anticipated to be able to promote osteogenesis. Whereas it still remains unknown whether it could enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In this study, GDY was modified with polyethylene glycol (PEG) and the influences of GDY-PEG at different concentrations on BMSCs cell growth and osteogenic differentiation were researched for the first time. In this study, we found that GDY-PEG at low concentration possessed premium bio-compatibility and revealed evident facilitation of BMSCs osteogenic differentiation. The cell growth and osteogenic differentiation of BMSCs treated with GDY-PEG were dose-dependent. GDY-PEG at 1 μg/mL demonstrated the optimal promoting effects of BMSCs osteogenic differentiation. Moreover, the regulating effect of BMSCs osteogenic differentiation by GDY-PEG might be associated with the Wnt/β-catenin signaling pathway. In all, the present study indicated a novel application of GDY in promoting bone tissue regeneration, providing a novel biomaterial for bone augmentation in clinics.
引导骨再生（GBR）是牙槽骨不足患者常用的技术。发现能够增强成骨作用的骨替代物对GBR至关重要。石墨炔（GDY）是一种新发现的碳基纳米材料，被认为是乙炔碳最稳定的同素异形体，有望促进成骨。然而，它是否能增强骨髓间充质干细胞（BMSC）的成骨分化仍不清楚。在本研究中，用聚乙二醇（PEG）对GDY进行修饰，并首次研究了不同浓度的GDY-PEG对BMSC细胞生长和成骨分化的影响。在本研究中，我们发现低浓度的GDY-PEG具有良好的生物相容性，并对BMSC的成骨分化有明显的促进作用。GDY-PEG处理的BMSC的细胞生长和成骨分化呈剂量依赖性。1μg/mL的GDY-PEG对BMSC的成骨分化具有最佳促进作用。此外，GDY-PEG对BMSC成骨分化的调节作用可能与Wnt/β-连环蛋白信号通路有关。总之，本研究表明GDY在促进骨组织再生方面有新的应用，为临床骨增量提供了一种新型生物材料。
