Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany.
BioInterface Group, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 52056 Aachen, Germany.
Int J Mol Sci. 2024 Mar 11;25(6):3207. doi: 10.3390/ijms25063207.
Periodontal defects' localization affects wound healing and bone remodeling, with faster healing in the upper jaw compared to the lower jaw. While differences in blood supply, innervation, and odontogenesis contribute, cell-intrinsic variances may exist. Few studies explored cell signaling in periodontal ligament stem cells (PDLSC), overlooking mandible-maxilla disparitiesUsing kinomics technology, we investigated molecular variances in PDLSC. Characterization involved stem cell surface markers, proliferation, and differentiation capacities. Kinase activity was analyzed via multiplex kinase profiling, mapping differential activity in known gene regulatory networks. Upstream kinase analysis identified stronger EphA receptor expression in the mandible, potentially inhibiting osteogenic differentiation. The PI3K-Akt pathway showed higher activity in lower-jaw PDLSC. PDLSC from the upper jaw exhibit superior proliferation and differentiation capabilities. Differential activation of gene regulatory pathways in upper vs. lower-jaw PDLSC suggests implications for regenerative therapies.
牙周缺陷的定位会影响伤口愈合和骨重塑，相较于下颌，上颌的愈合速度更快。虽然血液供应、神经支配和牙发生的差异有所贡献，但细胞内在的差异可能存在。少数研究探索了牙周韧带干细胞（PDLSC）中的细胞信号转导，忽略了下颌骨-上颌骨的差异。利用激酶组学技术，我们研究了 PDLSC 中的分子差异。特征在于干细胞表面标志物、增殖和分化能力。通过多激酶分析来分析激酶活性，描绘已知基因调控网络中的差异活性。上游激酶分析表明，下颌骨中 EphA 受体表达更强，可能抑制成骨分化。PI3K-Akt 通路在下颌 PDLSC 中表现出更高的活性。上颌 PDLSC 表现出更好的增殖和分化能力。上颌和下颌 PDLSC 中基因调控途径的差异激活表明其对再生治疗具有影响。
