通过重组痘苗病毒感染的小鼠细胞中乙型肝炎表面抗原的异源表达鉴定痘苗启动子。
Identification of vaccinia promoters by heterologous expression of hepatitis B surface antigen in mouse cells infected by recombinant vaccinia viruses.
作者信息
Barbeyron T, Mars M, Schroeder E, Malpièce Y, Plucienniczak A, Beaud G, Streeck R E
机构信息
Unité des Applications du Génie Génétique, Institut Pasteur, Paris, France.
出版信息
Biochim Biophys Acta. 1987 Dec 8;910(3):240-4. doi: 10.1016/0167-4781(87)90116-3.
DNA fragments preceding open reading frames in a conserved segment of the vaccinia virus genome (Plucienniczak A., et al. (1985) Nucleic Acids Res. 13, 985-998) were cloned into plasmids upstream of the S gene of the hepatitis B virus encoding the surface antigen (HBsAg). Recombinant vaccinia virus obtained after insertion of these constructs into the thymidine kinase gene were used to infect mouse 1D cells. HBsAg was assayed in cellular supernatants. A strong promoter was thus identified in a 295 bp fragment preceding the coding region of the 147 kDa subunit of the vaccinia RNA polymerase.
将痘苗病毒基因组保守区段中开放阅读框之前的DNA片段（Plucienniczak A.等人，(1985)《核酸研究》13卷，985 - 998页）克隆到编码表面抗原（HBsAg）的乙型肝炎病毒S基因上游的质粒中。将这些构建体插入胸苷激酶基因后获得的重组痘苗病毒用于感染小鼠1D细胞。在细胞上清液中检测HBsAg。因此，在痘苗病毒RNA聚合酶147 kDa亚基编码区之前的一个295 bp片段中鉴定出一个强启动子。
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