成功表达乙型肝炎表面抗原的改良重组LC16m0或LC16m8痘苗病毒。

Improved recombinant LC16m0 or LC16m8 vaccinia virus successfully expressing hepatitis B surface antigen.

作者信息

Watanabe K, Kobayashi H, Kajiyama K, Morita M, Yasuda A, Gotoh H, Saeki S, Sugimoto M, Saito H, Kojima A

机构信息

Chiba Serum Institute, Japan.

出版信息

Vaccine. 1989 Feb;7(1):53-9. doi: 10.1016/0264-410x(89)90011-x.

DOI:10.1016/0264-410x(89)90011-x

PMID:2718607

Abstract

An attempt was made to develop a safe, efficacious live recombinant vaccine using low neurovirulent strains of vaccinia virus: LC16m0 (m0) or LC16m8 (m8). Recombinant vaccinia virus (RVV) was constructed by inserting the hepatitis B surface antigen (HBsAg) gene fused to a 7.5 kDa protein promoter (7.5 kDa promoter) within the vaccinia virus thymidine kinase (TK) gene using the m0 or m8 strain as vectors. These RVVs expressed significant amounts of HBsAg (1.1 microgram/2 X 10(5) cells) consisting of 24.5 and 28 kDa (glycosylated) proteins. HBsAg produced by RVV (vaccinia HBsAg) had physical properties very similar to plasma-derived HBsAg. Considering the safety of RVVs from m0 or m8 that have been constructed with HBsAg without the addition of a promoter and the high induction of anti-HBs antibody with RVV from m0 strain in rabbits, the RVVs constructed in the present study are likely to form the basis of a safe live RVV vaccine for hepatitis B virus (HBV).

摘要

人们尝试利用低神经毒力的痘苗病毒株（LC16m0（m0）或LC16m8（m8））开发一种安全、有效的重组活疫苗。以m0或m8株为载体，通过将与7.5 kDa蛋白启动子（7.5 kDa启动子）融合的乙型肝炎表面抗原（HBsAg）基因插入痘苗病毒胸苷激酶（TK）基因中，构建重组痘苗病毒（RVV）。这些RVV表达了大量由24.5 kDa和28 kDa（糖基化）蛋白组成的HBsAg（1.1微克/2×10⁵个细胞）。RVV产生的HBsAg（痘苗HBsAg）的物理性质与血浆来源的HBsAg非常相似。考虑到未添加启动子构建的含HBsAg的m0或m8 RVV的安全性，以及m0株RVV在兔体内对抗HBs抗体的高诱导性，本研究构建的RVV可能构成一种安全的乙型肝炎病毒（HBV）重组活疫苗的基础。