Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.
Laboratorio de Ecología de Bosques Tropicales y Primatología, LEBTYP, Departamento de Ciencias Biológicas, Universidad de Los Andes, Cra. 1 N° 18a-12, 111711, Bogotá, Colombia.
J Wildl Dis. 2023 Jan 1;59(1):181-185. doi: 10.7589/JWD-D-22-00062.
Myiases are parasitic infections caused by the larval stages of some fly species. In American nonhuman primates (NHP), three bot fly species causing cutaneous myiasis have been reported: Cuterebra baeri, Cochliomyia hominivorax, and Dermatobia hominis. Studies on myiasis in NHP are scarce and mainly based on morphologic identification of larvae, while molecular approaches have been barely used. This study aimed to molecularly identify bot flies parasitizing two NHP species living sympatrically in central Colombia. Bot fly larvae were collected from two grey-legged night monkeys (Aotus griseimembra) and from a howler monkey (Alouatta seniculus). Larvae were measured and photographed for morphologic evaluation; subsequent molecular characterizations of a partial region of the cytochrome c oxidase subunit 1 mitochondrial gene were performed. Sequence analysis allowed the identification of all specimens as Cuterebra baeri, enabling confirmation of this species parasitizing Alouatta seniculus and Aotus griseimembra in Colombia.
蝇蛆病是由某些蝇类幼虫引起的寄生虫感染。在美国非人类灵长类动物（NHP）中，已经报道了三种引起皮肤蝇蛆病的狂蝇科物种：拔毛狂蝇、人肤蝇和皮肤蝇。关于 NHP 蝇蛆病的研究很少，主要基于幼虫的形态学鉴定，而分子方法几乎没有使用。本研究旨在对生活在哥伦比亚中部的两种共生的 NHP 物种寄生的狂蝇进行分子鉴定。从两只灰色夜猴（Aotus griseimembra）和一只吼猴（Alouatta seniculus）身上采集了狂蝇幼虫。对幼虫进行了测量和拍照，以进行形态学评估；随后对细胞色素 c 氧化酶亚基 1 线粒体基因的部分区域进行了分子特征分析。序列分析能够鉴定出所有标本均为拔毛狂蝇，从而证实该物种在哥伦比亚寄生在吼猴和灰色夜猴身上。
