Colwell D D, Milton K
Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
J Med Entomol. 1998 Sep;35(5):674-80. doi: 10.1093/jmedent/35.5.674.
The fecundity and development of larval stages of the cuterebrid bot fly A. baeri were studied in an unusual host, remotely related to the primary host. Third-instar Alouattamyia baeri (Shannon & Greene) removed from howler monkeys, Alouatta palliata, were allowed to pupate and then were cultured under controlled conditions. Eclosion occurred after 37.9 +/- 0.4 (mean +/- SE) (male) and 38.2 +/- 0.4 (female) d at 26 degrees C. Five-day-old females were mated using a tethered flight technique and oviposited on ridged filter paper. The total egg complement was 1,399 +/- 243 (n = 2) eggs per female. Eggs were fully embryonated after incubation for 5 d at 26 degrees C. Eggs hatched when warmed in the palm of the hand. Hatching of eggs from an individual batch was asynchronous. Newly hatched larvae would not penetrate intact skin on a rabbit, Oryctolagus cuniculus L. Larvae placed near the nares or on the ocular conjunctiva migrated rapidly from view. Warbles containing larvae were first observed on infested rabbits 5 d after infestation. Development of larvae proceeded until day 39 after infestation, when nearly mature 3rd instars were observed. None of the larvae survived to pupate.
在一种与主要宿主亲缘关系较远的不寻常宿主中，研究了巴氏皮肤蝇幼虫阶段的繁殖力和发育情况。从吼猴（蛛猴）身上取出的三龄巴氏皮肤蝇（香农和格林），让其化蛹，然后在可控条件下进行培养。在26摄氏度下，雄性在37.9±0.4（平均值±标准误）天、雌性在38.2±0.4天羽化。5日龄的雌性采用系留飞行技术进行交配，并在有脊的滤纸上产卵。每只雌性的总卵量为1399±243（n = 2）枚。卵在26摄氏度下孵化5天后完全胚胎化。卵在手掌中温热时孵化。同一批卵的孵化是不同步的。新孵化的幼虫不会穿透家兔完整的皮肤。放置在鼻孔附近或眼结膜上的幼虫会迅速从视野中消失。在感染后5天，首次在受感染的家兔身上观察到含有幼虫的疖肿。幼虫发育一直持续到感染后第39天，此时观察到接近成熟的三龄幼虫。没有幼虫存活到化蛹阶段。
