Antibody immunoglobulin G (IgG) response to Alouattamyia baeri (Diptera:Cuterebridae) parasitism of howler monkeys, Alouatta palliata, in Panama.

Larval bot fly burdens and the presence of immunoglobulin G (IgG) antibodies to larval antigens of Alouattamyia baeri (Shannon & Greene) were determined in howler monkeys, Alouatta palliata authority, from Barro Colorado Island, Panama, during July and August of 1991 and 1992. Monkeys produced antibodies (IgG) to both 1st- and 3rd-instar proteins of the monkey bot as measured by an enzyme immunoassay. The response to 1st-instar antigen was correlated with number of bots for the 1991 data and for pooled data from 1991 and 1992. No correlation was observed for the response to 3rd instar antigen. First-instar extracts were composed of 9 major proteins as visualized by SDS-PAGE. Bands at 17, 25, and 32 kDa were positive in Western blots. Third-instar extracts contained at least 13 major bands, with those at 120 and 130 kDa reactive in immunoblots. The immune response to A. baeri may be involved in limiting larval bot numbers.