Baron R W, Colwell D D, Milton K
Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
J Med Entomol. 1996 Nov;33(6):946-51. doi: 10.1093/jmedent/33.6.946.
Larval bot fly burdens and the presence of immunoglobulin G (IgG) antibodies to larval antigens of Alouattamyia baeri (Shannon & Greene) were determined in howler monkeys, Alouatta palliata authority, from Barro Colorado Island, Panama, during July and August of 1991 and 1992. Monkeys produced antibodies (IgG) to both 1st- and 3rd-instar proteins of the monkey bot as measured by an enzyme immunoassay. The response to 1st-instar antigen was correlated with number of bots for the 1991 data and for pooled data from 1991 and 1992. No correlation was observed for the response to 3rd instar antigen. First-instar extracts were composed of 9 major proteins as visualized by SDS-PAGE. Bands at 17, 25, and 32 kDa were positive in Western blots. Third-instar extracts contained at least 13 major bands, with those at 120 and 130 kDa reactive in immunoblots. The immune response to A. baeri may be involved in limiting larval bot numbers.
1991年7月至8月以及1992年7月至8月期间，在巴拿马巴罗科罗拉多岛的鬃毛吼猴（Alouatta palliata authority）身上，测定了幼虫肤蝇的感染量以及针对巴氏肤蝇（Alouattamyia baeri，香农和格林命名）幼虫抗原的免疫球蛋白G（IgG）抗体的存在情况。通过酶免疫测定法检测发现，猴子针对猴肤蝇一龄幼虫和三龄幼虫的蛋白质均产生了抗体（IgG）。对于1991年的数据以及1991年和1992年的汇总数据，猴子对一龄幼虫抗原的反应与肤蝇数量相关。而对于三龄幼虫抗原的反应，未观察到相关性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）可见，一龄幼虫提取物由9种主要蛋白质组成。在蛋白质印迹法中，17、25和32千道尔顿（kDa）处的条带呈阳性。三龄幼虫提取物至少包含13条主要条带，在免疫印迹法中，120和130 kDa处的条带具有反应性。对巴氏肤蝇的免疫反应可能参与限制幼虫肤蝇的数量。
