Decombe Alice, El Kazzi Priscila, Decroly Etienne
AFMB, CNRS, Aix-Marseille University, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France.
AFMB, CNRS, Aix-Marseille University, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France.
Curr Opin Virol. 2023 Apr;59:101302. doi: 10.1016/j.coviro.2023.101302. Epub 2023 Feb 8.
Viral RNAs (vRNAs) are decorated by post-transcriptional modifications, including methylation of nucleotides. Methylations regulate biological functions linked to the sequence, structure, and protein interactome of RNA. Several RNA viruses were found to harbor 2'-O-methylations, affecting the ribose moiety of RNA. This mark was initially shown to target the first and second nucleotides of the 5'-end cap structure of mRNA. More recently, nucleotides within vRNA were also reported to carry 2'-O-methylations. The consequences of such methylations are still puzzling since they were associated with both proviral and antiviral effects. Here, we focus on the mechanisms governing vRNA 2'-O-methylation and we explore the possible roles of this epitranscriptomic modification for viral replication.
病毒RNA（vRNA）会进行转录后修饰，包括核苷酸甲基化。甲基化调控与RNA的序列、结构和蛋白质相互作用组相关的生物学功能。已发现几种RNA病毒含有2'-O-甲基化修饰，影响RNA的核糖部分。该修饰最初被证明靶向mRNA 5'-端帽结构的第一个和第二个核苷酸。最近，也有报道称vRNA中的核苷酸也带有2'-O-甲基化修饰。这种甲基化修饰的后果仍然令人困惑，因为它们既与病毒促进作用有关，也与抗病毒作用有关。在这里，我们专注于调控vRNA 2'-O-甲基化的机制，并探讨这种表观转录组修饰在病毒复制中的可能作用。
