Department of Dentistry, Omsk State Medical University, Omsk, Russian Federation.
Department of the Organization of the Dental Service, Licensing and Accreditation, Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russian Federation.
Appl Spectrosc. 2023 May;77(5):457-469. doi: 10.1177/00037028231160145. Epub 2023 Feb 24.
The aim of this study was to research the biochemical changes in the hard tissues of the lower "wisdom" teeth, which are at the stage of eruption, with connective tissue dysplasia, depending on sex. The study involved 38 patients aged 17-25 years, who had the extraction of the lower third molars on the left and right for medical reasons. Twenty-one participants in the experiment (16 females, five males) were diagnosed with connective tissue dysplasia; the remaining 17 were healthy and made up the control group (11 females, six males). Samples of enamel, dentin, and enamel-dentin junction were dried to constant weight and examined in tablets pressed in a mixture with potassium bromide on an FT-801 Fourier transform infrared (FT-IR) spectrometer (NPF Simex, Russia) in the range of 500-4000 cm. In the IR spectra of the enamel, enamel-dentin junction, and dentin, the absorbance ratio of amide I and II changed significantly and the absorbance of the absorption band of phosphate ions decreased from enamel to dentin. Differences between groups with and without connective tissue dysplasia increased in the following series: dentin, enamel-dentin junction, and enamel. With connective tissue dysplasia, a change in the ratio of the intensities of the absorption bands of amide I and phosphate ions was observed. Normally, the content of organic substances in the enamel of the teeth in the males group is lower than in the females group. Against the background of connective tissue dysplasia, the content of organic substances in the enamel in women decreased while in men it increased, which confirmed the presence of sexual dimorphism during the maturation of hard dental tissues at the eruption stage. The study confirms the opinion of many researchers about the significant role of collagen proteins, which, together with non-collagen proteins, are involved in the development and maturation of dental hard tissues, which are characterized by pronounced heterogeneity and sexual dimorphism in the group with connective tissue dysplasia.
本研究旨在研究处于萌出阶段且伴有结缔组织发育不良的下颌“智齿”的硬组织的生化变化，并根据性别进行研究。该研究纳入了 38 名 17-25 岁因医疗原因接受左右下颌第三磨牙拔除的患者。21 名实验参与者（16 名女性，5 名男性）被诊断为结缔组织发育不良；其余 17 名参与者健康，构成对照组（11 名女性，6 名男性）。将釉质、牙本质和釉牙本质交界处的样本干燥至恒重，并在 FT-801 傅里叶变换红外（FT-IR）光谱仪（NPF Simex，俄罗斯）上用与溴化钾混合压制成片剂进行检查，在 500-4000 cm 的范围内进行检查。在釉质、釉牙本质交界处和牙本质的红外光谱中，酰胺 I 和 II 的吸光度比发生了显著变化，并且从釉质到牙本质，磷酸离子吸收带的吸光度降低。在有和没有结缔组织发育不良的组之间，以下系列的差异增加：牙本质、釉牙本质交界处和釉质。在伴有结缔组织发育不良的情况下，观察到酰胺 I 和磷酸离子吸收带强度比的变化。通常，男性组牙齿釉质中的有机物质含量低于女性组。在结缔组织发育不良的背景下，女性牙齿釉质中的有机物质含量减少，而男性则增加，这证实了在硬组织成熟阶段的萌出阶段存在性别二态性。该研究证实了许多研究人员的观点，即胶原蛋白的重要作用，胶原蛋白与非胶原蛋白一起参与牙硬组织的发育和成熟，在伴有结缔组织发育不良的组中表现出明显的异质性和性别二态性。
