[Study on the Regulation of Chidamide on CD8T Cells in T-cell Acute Lymphoblastic Leukemia].

OBJECTIVE

To explore the regulatory effect of chidamide on CD8 T cells in T-cell acute lymphoblastic leukemia.

METHODS

The expression levels of and mRNA in Jurkat cells, lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells were detected by fluorescence quantitative PCR. The proportion of CD8 T cells in lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells was determined by flow cytometry.

RESULTS

Chidamide upregulated mRNA expression in Jurkat cell line in a dose-dependent manner (r=0.950). The mRNA expression of in chidamide 5 μmol/L group was 164 times higher than that in control group. Chidamide upregulated mRNA expression in lymphocytes, but the up-regulated level was significantly lower than that in Jurkat cell line treated with the same concentration of chidamide. Co-culture with chidamide treated Jurkat cells upregulated the proportion of CD8 T cells in lymphocytes.

CONCLUSION

In T-cell acute lymphoblastic leukemia, chidamide may increase the concentration of CXCL9 in the tumor microenvironment by up-regulating the expression of CXCL9 in tumor cells, leading to an increase in the number of CD8 T cells.