Yan Miao-Ming, Li Zhao-Xuan, Chen Chong, Zhang Wei, Zhou Dao-Bin
Department of Hematology, Peking Union Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Department of Immunology, Institute of Basic Medical Science CAMS, School of Basic Medicine PUMC, Beijing 100005, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Feb;31(1):71-75. doi: 10.19746/j.cnki.issn.1009-2137.2023.01.011.
To explore the regulatory effect of chidamide on CD8 T cells in T-cell acute lymphoblastic leukemia.
The expression levels of and mRNA in Jurkat cells, lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells were detected by fluorescence quantitative PCR. The proportion of CD8 T cells in lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells was determined by flow cytometry.
Chidamide upregulated mRNA expression in Jurkat cell line in a dose-dependent manner (r=0.950). The mRNA expression of in chidamide 5 μmol/L group was 164 times higher than that in control group. Chidamide upregulated mRNA expression in lymphocytes, but the up-regulated level was significantly lower than that in Jurkat cell line treated with the same concentration of chidamide. Co-culture with chidamide treated Jurkat cells upregulated the proportion of CD8 T cells in lymphocytes.
In T-cell acute lymphoblastic leukemia, chidamide may increase the concentration of CXCL9 in the tumor microenvironment by up-regulating the expression of CXCL9 in tumor cells, leading to an increase in the number of CD8 T cells.
探讨西达本胺对T细胞急性淋巴细胞白血病中CD8⁺ T细胞的调控作用。
采用荧光定量PCR检测西达本胺处理的Jurkat细胞、淋巴细胞以及与经西达本胺处理的Jurkat细胞共培养的淋巴细胞中CXCL9和CXCR3 mRNA的表达水平。采用流式细胞术检测西达本胺处理的淋巴细胞以及与经西达本胺处理的Jurkat细胞共培养的淋巴细胞中CD8⁺ T细胞的比例。
西达本胺呈剂量依赖性上调Jurkat细胞系中CXCL9 mRNA表达（r = 0.950）。西达本胺5 μmol/L组CXCL9 mRNA表达水平较对照组高164倍。西达本胺上调淋巴细胞中CXCL9 mRNA表达，但上调水平明显低于相同浓度西达本胺处理的Jurkat细胞系。与经西达本胺处理的Jurkat细胞共培养上调淋巴细胞中CD8⁺ T细胞比例。
在T细胞急性淋巴细胞白血病中，西达本胺可能通过上调肿瘤细胞中CXCL9的表达，增加肿瘤微环境中CXCL9浓度，从而导致CD8⁺ T细胞数量增加。
