Paris Elizabeth A, Bahr Janice M, Basu Sanjib, Barua Animesh
Department of Anatomy & Cell Biology, Rush University Medical Center, Chicago, IL 60612, USA.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Cancers (Basel). 2023 Jan 21;15(3):661. doi: 10.3390/cancers15030661.
Ovarian high-grade serous carcinoma (HGSC) is a fatal malignancy of women. Alterations in the expression of nuclear proteins are early steps in malignant transformation; nucleolin is one such protein. Changes in nucleolin expression and circulatory levels during ovarian HGSC development are unknown. The study goal was to determine if tissue and circulatory levels of nucleolin change in response to malignant transformation leading to ovarian HGSC.
Sera, ovaries, and BRCA+ fimbria from healthy subjects, and sera and tumor tissues from patients ( = 10 each), and healthy hens and hens with HGSC were examined in exploratory and prospective studies for nucleolin expression by immunohistochemistry, immunoblotting, gene expression, and immunoassay, and analyzed by analysis of variance (ANOVA).
Compared with normal, nucleolin expression was higher in patients and hens with ovarian HGSC and in women with a risk of HGSC ( < 0.05). Compared with normal (1400 + 105 pg/mL, = 8), serum nucleolin levels were 1.5 and 1.7-fold higher in patients with early- ( = 5) and late-stage ( = 5) HGSC, respectively. Additionally, serum nucleolin levels increased significantly ( < 0.05) prior to the formation of detectable masses.
This pilot study concluded that tissue and serum levels of nucleolin increase in association with malignant changes in ovaries and fimbriae leading to ovarian HGSC.
卵巢高级别浆液性癌（HGSC）是一种致命的女性恶性肿瘤。核蛋白表达的改变是恶性转化的早期步骤；核仁素就是这样一种蛋白。卵巢HGSC发生过程中核仁素表达及循环水平的变化尚不清楚。本研究的目的是确定核仁素的组织和循环水平是否会因导致卵巢HGSC的恶性转化而发生变化。
在探索性和前瞻性研究中，通过免疫组织化学、免疫印迹、基因表达和免疫测定法检测健康受试者的血清、卵巢和BRCA+输卵管伞，以及患者（每组10例）的血清和肿瘤组织，还有健康母鸡和患有HGSC的母鸡的核仁素表达，并通过方差分析（ANOVA）进行分析。
与正常情况相比，卵巢HGSC患者和母鸡以及有HGSC风险的女性的核仁素表达更高（P<0.05）。与正常情况（1400 + 105 pg/mL，n = 8）相比，早期HGSC患者（n = 5）和晚期HGSC患者（n = 5）的血清核仁素水平分别高出1.5倍和1.7倍。此外，在可检测肿块形成之前，血清核仁素水平显著升高（P<0.05）。
这项初步研究得出结论，核仁素的组织和血清水平随着卵巢和输卵管伞向卵巢HGSC的恶性变化而增加。
