Chamani Faraz, Pyle Marla M, Shrestha Tej B, Sebek Jan, Bossmann Stefan H, Basel Matthew T, Sheth Rahul A, Prakash Punit
Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS 66506, USA.
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Cancers (Basel). 2023 Jan 21;15(3):655. doi: 10.3390/cancers15030655.
Thermal therapies are under investigation as part of multi-modality strategies for the treatment of pancreatic cancer. In the present study, we determined the kinetics of thermal injury to pancreatic cancer cells in vitro and evaluated predictive models for thermal injury. Cell viability was measured in two murine pancreatic cancer cell lines (KPC, Pan02) and a normal fibroblast (STO) cell line following in vitro heating in the range 42.5-50 °C for 3-60 min. Based on measured viability data, the kinetic parameters of thermal injury were used to predict the extent of heat-induced damage. Of the three thermal injury models considered in this study, the Arrhenius model with time delay provided the most accurate prediction (root mean square error = 8.48%) for all cell lines. Pan02 and STO cells were the most resistant and susceptible to hyperthermia treatments, respectively. The presented data may contribute to studies investigating the use of thermal therapies as part of pancreatic cancer treatment strategies and inform the design of treatment planning strategies.
热疗法作为胰腺癌多模式治疗策略的一部分正在研究中。在本研究中，我们确定了体外胰腺癌细胞热损伤的动力学，并评估了热损伤的预测模型。在42.5-50°C范围内体外加热3-60分钟后，测量了两种小鼠胰腺癌细胞系（KPC、Pan02）和一种正常成纤维细胞（STO）细胞系的细胞活力。基于测得的活力数据，热损伤的动力学参数用于预测热诱导损伤的程度。在本研究考虑的三种热损伤模型中，具有时间延迟的阿伦尼乌斯模型对所有细胞系提供了最准确的预测（均方根误差=8.48%）。Pan02和STO细胞分别对热疗最具抗性和敏感性。所呈现的数据可能有助于研究将热疗法作为胰腺癌治疗策略的一部分，并为治疗计划策略的设计提供信息。
