Valero Yulema, Mercado Luis, Arizcun Marta, Cuesta Alberto, Chaves-Pozo Elena
Oceanographic Centre of Murcia, Spanish Institute of Oceanography, Spanish National Research Council, Carretera de la Azohía s/n, Puerto de Mazarrón, 30860 Murcia, Spain.
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2362807, Chile.
Animals (Basel). 2023 Jan 26;13(3):415. doi: 10.3390/ani13030415.
Acquiring immunocompetence is essential in the development of fish embryos, as they are exposed to environmental pathogens even before they are fertilized. Despite the importance of the antimicrobial function as the first line of defense against foreign microorganisms, little knowledge is available about its role in larval development. In vertebrates, transgenerational immune priming influences the acquisition of immunocompetence of specimens, regulating the selective allocation of nongenetic resources to their progeny and modulating their development. In this work, we primed teleost European sea bass broodstock females with a viral protein expression vector in order to evaluate the innate immunity development of their offspring. Several antimicrobial functions, the pattern of expression of gene coding for different antimicrobial peptides (AMPs), and their protein levels, were evaluated in eggs and larvae during development. Our data determined the presence of antimicrobial proteins of maternal origin in eggs, and that female vaccination increases antimicrobial activities and the transcription and synthesis of AMPs during larval development.
获得免疫能力在鱼类胚胎发育过程中至关重要，因为它们甚至在受精之前就暴露于环境病原体中。尽管抗菌功能作为抵御外来微生物的第一道防线很重要，但关于其在幼体发育中的作用却知之甚少。在脊椎动物中，跨代免疫启动会影响样本免疫能力的获得，调节非遗传资源向其后代的选择性分配并调节其发育。在这项工作中，我们用病毒蛋白表达载体对硬骨鱼欧洲鲈鱼亲鱼雌性进行了启动，以评估其后代的先天免疫发育。在发育过程中，对卵和幼体中的几种抗菌功能、编码不同抗菌肽（AMPs）的基因的表达模式及其蛋白质水平进行了评估。我们的数据确定了卵中存在母源抗菌蛋白，并且雌性疫苗接种会增加幼体发育过程中的抗菌活性以及AMPs的转录和合成。
