Oceanographic Center of Murcia, Spanish Institute of Oceanography (IEO), Carretera de la Azohía s/n. 30860, Puerto de Mazarrón, Murcia, Spain.
Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100, Murcia, Spain.
Sci Rep. 2020 May 14;10(1):7966. doi: 10.1038/s41598-020-64522-2.
17α-ethynilestradiol (EE) and tamoxifen (Tmx) are pollutants world-wide distributed in aquatic environments. Gilthead seabream, Sparus aurata L., is highlighted as a species model of intensively culture in anthropogenic disturbed environments. The effects of these pollutants on gilthead seabream reproduction and some immune responses have been described but, the humoral innate antimicrobial activities have never received attention. In this work we analysed the latest in the plasma of gilthead seabream males of different ages and reproductive stages treated with 0, 2.5, 5 or 50 μg EE or 100 μg Tmx g food during different times of exposure and of reverting to commercial diet (recovery). The peroxidase and protease activities decreased as the spermatogenesis of the first reproductive cycle (RC) proceeded in control fish. However, only protease and antiprotease activities showed different level at different stages of the second RC in control fish, but showed scarce disruption in fish treated with EE or Tmx. Peroxidase and bactericide activities are more sensitive to EE, than to Tmx. The effects induced by EE varied depending on the activity analyzed, the dose and the time of exposure and the reproductive stage and the age of the specimens.
17α-乙炔基雌二醇（EE）和他莫昔芬（Tmx）是在水生环境中广泛分布的世界性污染物。金头鲷（Sparus aurata L.）被突出为一种在人为干扰环境中集约化养殖的物种模式。这些污染物对金头鲷繁殖和一些免疫反应的影响已经被描述过，但体液先天抗菌活性从未受到关注。在这项工作中，我们分析了不同年龄和生殖阶段的雄性金头鲷在暴露于 0、2.5、5 或 50μg EE 或 100μg Tmx g 食物不同时间以及恢复商业饮食（恢复）后的血浆中的最新情况。在对照组鱼中，随着第一次生殖周期（RC）的精子发生，过氧化物酶和蛋白酶活性降低。然而，只有蛋白酶和抗蛋白酶活性在对照组鱼的第二次 RC 的不同阶段表现出不同的水平，但在 EE 或 Tmx 处理的鱼中几乎没有受到干扰。过氧化物酶和杀菌剂活性对 EE 的敏感性高于 Tmx。EE 诱导的影响取决于所分析的活性、剂量、暴露时间以及标本的生殖阶段和年龄。
