Headley Selwyn Arlington, Dall Agnol Alais Maria, Oliveira Thalita Evani Silva, Bon Vinícius Rodrigues, Scuisato Gabriela Sanches, Xavier Ana Aparecida Correa, Yasumitsu Carolina Yuka, Alfieri Alice Fernandes, Alfieri Amauri Alcindo
Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paraná, Brazil.
Multi-User Animal Health Laboratory, Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paraná, Brazil.
Animals (Basel). 2023 Jan 26;13(3):417. doi: 10.3390/ani13030417.
Bovine gammaherpesvirus 6 (BoGHV6), previously known as bovine lymphotropic virus, is a member of the genus, subfamily Gammaherpesvirinae. Other members of the genus include viruses that produce malignant catarrhal fever (MCF) in mammalian hosts, collectively referred to as the MCF virus (MCFV) complex, and the porcine lymphotropic herpesvirus (PLHV). However, the current role of BoGHV6 in the development of diseases and/or disease syndromes remains uncertain and controversial. This paper investigated the participation of BoGHV6 in the development of pulmonary disease in a cow with interstitial pneumonia by histopathology and molecular testing. Tissue antigens of common viral agents of respiratory diseases and were not identified by immunohistochemistry. Additionally, molecular assays designed to amplify common bacterial and viral pathogens of pulmonary disease did not amplify the nucleic acids of these agents. However, a pan-PCR assay amplified the DNA of the herpesvirus polymerase gene, while the specific BoGHV6 nested-PCR assay amplified the partial fragment of the BoGHV6 polymerase gene derived from the pulmonary tissue with interstitial pneumonia. Phylogenetic analysis revealed that the BoGHV6 strain herein identified had 99.8% nucleotide (nt) sequence identity with reference strains of BoGHV6, but only 72.2-73.5% and 67.9-68.6% nt identity with reference strains of MCFV and PLHV, respectively. Consequently, these results suggest that BoGHV6 was associated with the pulmonary disease observed in this cow.
牛γ疱疹病毒6型（BoGHV6），以前称为牛嗜淋巴细胞病毒，是γ疱疹病毒亚科中的一个属的成员。该属的其他成员包括在哺乳动物宿主中引发恶性卡他热（MCF）的病毒，统称为MCF病毒（MCFV）复合体，以及猪嗜淋巴细胞疱疹病毒（PLHV）。然而，BoGHV6在疾病和/或疾病综合征发展中的当前作用仍不确定且存在争议。本文通过组织病理学和分子检测研究了BoGHV6在一头患有间质性肺炎的奶牛肺部疾病发展中的参与情况。通过免疫组织化学未鉴定出呼吸道疾病常见病毒病原体的组织抗原。此外，旨在扩增肺部疾病常见细菌和病毒病原体的分子检测未扩增出这些病原体的核酸。然而，一种泛PCR检测扩增出了疱疹病毒聚合酶基因的DNA，而特异性BoGHV6巢式PCR检测扩增出了源自患有间质性肺炎的肺部组织的BoGHV6聚合酶基因的部分片段。系统发育分析表明，本文鉴定的BoGHV6毒株与BoGHV6参考毒株具有99.8%的核苷酸（nt）序列同一性，但与MCFV和PLHV参考毒株的nt同一性分别仅为72.2 - 73.5%和67.9 - 68.6%。因此，这些结果表明BoGHV6与该奶牛观察到的肺部疾病有关。
