Kim Jong-Hoon, Ku Bon-Hwan, Ko Gwang-Pyo, Kang Man-Jong, Son Kwang-Hee, Bang Mi-Ae, Park Ho-Yong
Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Insect Biotech Co., Ltd., Daejeon 34054, Republic of Korea.
Animals (Basel). 2023 Jan 26;13(3):423. doi: 10.3390/ani13030423.
The supplementation of pig diets with exogenous enzymes is widely used with the expectation that it will improve the efficiency of nutrient utilization, thereby, improving growth performance. This study aims to evaluate the effects of a 0.1% (/) multi-enzyme (a mixture of arazyme (2,500,000 Unit/kg), xylanase (200,000 Unit/kg) and mannanase (200,000 Unit/kg)) supplementation derived from invertebrate symbiotic bacteria on pig performance. Here, 256 growing pigs were assigned to control and treatment groups, respectively. The treatment group exhibited a significantly reduced average slaughter age; the final body weight and average daily gain increased compared with that of the control group. In the treatment group, the longissimus muscle showed a remarkable decrease in cooking loss, shear force, and color values with increased essential and non-essential amino acid concentrations. Furthermore, the concentrations of mono- and polyunsaturated fatty acids in the treatment group increased. Feed additive supplementation increased the family of Ruminococcaceae and genera , , , and , which play a positive role in the host physiology and health. Predicted metabolic pathway analysis confirmed that operational taxonomic units and predicted amino acid biosynthesis pathways were strongly associated. The results suggest that applying exogenous enzymes derived from invertebrate symbiotic bacteria enhances animal performance.
在猪日粮中添加外源酶被广泛应用，期望能提高养分利用效率，从而改善生长性能。本研究旨在评估添加0.1%（/）源自无脊椎动物共生细菌的复合酶（一种由阿拉伯聚糖酶（2,500,000单位/千克）、木聚糖酶（200,000单位/千克）和甘露聚糖酶（200,000单位/千克）组成的混合物）对猪生产性能的影响。在此，256头生长猪分别被分配到对照组和处理组。处理组的平均屠宰年龄显著降低；与对照组相比，最终体重和平均日增重增加。在处理组中，随着必需和非必需氨基酸浓度的增加，背最长肌的蒸煮损失、剪切力和颜色值显著降低。此外，处理组中饱和脂肪酸和多不饱和脂肪酸的浓度增加。添加饲料添加剂增加了瘤胃球菌科以及属、、、和的丰度，它们对宿主生理和健康发挥着积极作用。预测代谢途径分析证实，操作分类单元与预测的氨基酸生物合成途径密切相关。结果表明，应用源自无脊椎动物共生细菌的外源酶可提高动物生产性能。
