Jiao Anran, Diao Hui, Yu Bing, He Jun, Yu Jie, Zheng Ping, Luo Yuheng, Luo Junqiu, Wang Quyuan, Wang Huifen, Mao Xiangbing, Chen Daiwen
Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key Laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Ya'an, 625014, China.
Anim Nutr. 2021 Mar;7(1):94-100. doi: 10.1016/j.aninu.2020.05.009. Epub 2020 Oct 22.
Short chain fatty acids (SCFA) are the main products of indigestible carbohydrates undergoing bacterial fermentation in the hindgut, which are related to some physiological functions. This study was designed to investigate the effects of SCFA infusion by ileum on the carcass traits, meat quality and lipid metabolism of growing pigs. In a 28-day study, 24 growing barrows fitted with a T-cannula in distal ileum were divided into 4 treatments: 1) Control, 2) antibiotics (AB), 3) AB + 300 mL of SCFA1 solution (ABS1), 4) AB + 300 mL of SCFA2 solution (ABS2). The concentrations of acetate, propionate and butyrate in SCFA1 solution were respectively 61.84, 18.62 and 12.55 mmol/L, and in SCFA2 were respectively 40.08, 15.41 and 9.78 mmol/L. The results showed that the SCFA infusion increased the average daily feed intake and average daily gain of pigs ( 0.05). Meanwhile, the SCFA treatments increased longissimus dorsi area ( 0.05) and carcass weight ( = 0.058), decreased the drip loss of longissimus dorsi ( = 0.059), and reduced serum concentrations of triglyceride, total cholesterol and urea nitrogen ( 0.05). Besides, the SCFA administration inhibited the mRNA expressions of fatty acid synthase () and acetyl-CoA carboxylase in longissimus dorsi ( 0.05), the mRNA expression of in the liver ( 0.05), and the mRNA expression of hormone-sensitive lipase in abdominal fat ( 0.05). Short chain fatty acid infusion also enhanced the mRNA expression of carnitine palmitoyltransferase-1α in the liver ( 0.05), the mRNA expressions of peroxisome proliferator activated receptor gamma and lipoprotein lipase in abdominal fat ( 0.05), and the mRNA expressions of free fatty acid receptor 2, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon ( 0.05). These results suggested that SCFA administration in the ileum could improve the carcass traits and meat quality of growing pigs, which was possibly due to the fact that SCFA modulated lipid metabolism.
短链脂肪酸（SCFA）是后肠中难消化碳水化合物经细菌发酵产生的主要产物，与某些生理功能相关。本研究旨在探讨回肠灌注短链脂肪酸对生长猪胴体性状、肉质和脂质代谢的影响。在一项为期28天的研究中，将24头在回肠末端安装了T型套管的生长公猪分为4组处理：1）对照组，2）抗生素组（AB），3）抗生素+300 mL SCFA1溶液组（ABS1），4）抗生素+300 mL SCFA2溶液组（ABS2）。SCFA1溶液中乙酸、丙酸和丁酸的浓度分别为61.84、18.62和12.55 mmol/L，SCFA2溶液中分别为40.08、15.41和9.78 mmol/L。结果表明，灌注短链脂肪酸提高了猪的平均日采食量和平均日增重（P<0.05）。同时，短链脂肪酸处理增加了背最长肌面积（P<0.05）和胴体重（P=0.058），降低了背最长肌的滴水损失（P=0.059），并降低了血清甘油三酯、总胆固醇和尿素氮的浓度（P<0.05）。此外，施用短链脂肪酸抑制了背最长肌中脂肪酸合酶（FAS）和乙酰辅酶A羧化酶的mRNA表达（P<0.05）、肝脏中FAS的mRNA表达（P<0.05）以及腹部脂肪中激素敏感性脂肪酶的mRNA表达（P<0.05）。灌注短链脂肪酸还增强了肝脏中肉碱棕榈酰转移酶-1α的mRNA表达（P<0.05）、腹部脂肪中过氧化物酶体增殖物激活受体γ和脂蛋白脂肪酶的mRNA表达（P<0.05）以及结肠中游离脂肪酸受体2、葡萄糖-6-磷酸酶和磷酸烯醇式丙酮酸羧激酶1的mRNA表达（P<0.05）。这些结果表明，回肠施用短链脂肪酸可改善生长猪的胴体性状和肉质，这可能是由于短链脂肪酸调节了脂质代谢。
