Parisi Francesca, Millanta Francesca, Nicastro Marika, Vannozzi Iacopo, Poli Alessandro
Department of Veterinary Sciences, University of Pisa, Viale Delle Piagge n. 2, 56124 Pisa, Italy.
Animals (Basel). 2023 Jan 31;13(3):505. doi: 10.3390/ani13030505.
Foxp3+ cell counts were evaluated by immunohistochemistry in 59 canine mammary tumors, 20 adenomas, and 39 carcinomas in three different compartments: intratumoral, within the adjacent stroma, and in the distant stroma. Foxp3+ lymphocyte counts were compared with histotype, grading, presence of lymphatic invasion, immunohistochemical expression of estrogen and progesterone receptors, expression of c-erbB-2, and the overall survival (OS). Our findings confirmed that Foxp3+ cells were significantly higher in canine mammary carcinomas compared to adenomas. A significantly higher number of Foxp3+ cells were detected in grade III carcinomas compared to grade II carcinomas, as well as in tumors with lymphatic invasion and loss of ER-expression. Finally, a high number of Foxp3+ cells was associated with poor prognosis. In conclusion, our findings highlighted the association of Foxp3+ lymphocytes with negative clinicopathological features and shorter overall survival (OS), thus confirming the role of Tregs as a negative prognostic marker in canine mammary carcinomas.
通过免疫组织化学方法，在59例犬乳腺肿瘤、20例腺瘤和39例癌中，对三个不同区域（瘤内、相邻间质和远处间质）的Foxp3 +细胞计数进行了评估。将Foxp3 +淋巴细胞计数与组织学类型、分级、淋巴管浸润情况、雌激素和孕激素受体的免疫组化表达、c-erbB-2的表达以及总生存期（OS）进行了比较。我们的研究结果证实，与腺瘤相比，犬乳腺癌中的Foxp3 +细胞明显更多。与II级癌相比，III级癌以及伴有淋巴管浸润和雌激素受体表达缺失的肿瘤中，检测到的Foxp3 +细胞数量明显更多。最后，大量的Foxp3 +细胞与预后不良相关。总之，我们的研究结果突出了Foxp3 +淋巴细胞与负面临床病理特征及较短总生存期（OS）之间的关联，从而证实了调节性T细胞作为犬乳腺癌阴性预后标志物的作用。
