Rodríguez Francisco, Rosales Rubén S, Ramírez Ana S, Poveda José B
Instituto Universitario de Sanidad Animal y Seguridad Alimentaria, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña S/N, 35413 Arucas, Spain.
Animals (Basel). 2023 Feb 1;13(3):520. doi: 10.3390/ani13030520.
(Mhy) is the causative agent of enzootic pneumonia, characterized by high morbidity and low mortality rates in intensive swine production systems. To better understand the mechanisms underlying the protection of an inactivated whole cell vaccine, we investigated the immunohistochemical differences in the cytokine expression in vaccinated and non-vaccinated pigs experimentally infected with Mhy. Four-week-old Mhy-negative pigs ( = 24) were allocated to negative control ( = 8), or one of two Mhy-infected groups: vaccinated ( = 8) and non-vaccinated ( = 8). Infection was carried out by a combination of trans-tracheal and aerosol route. Lung samples were processed for histopathological and immunohistochemical studies, by using antibodies against Mhy, IL1-α, IL1-β, IL-2, IL-4, IL-5, IL-6, Il-8, IL-10, IL-12p35, IL-13, IL-17A, TNF-α, IFN-γ, and CD-4 lymphocytes. Although all cytokines increased in both infected groups, IL-2, IL-12, and IFN-γ were significantly overexpressed in vaccinated pigs. These findings, in conjunction with the decrease of macroscopic and histological lesions in vaccinated animals, indicate the importance to enhance Th1 response in the immunization strategies to control Mhy infection.
猪肺炎支原体（Mhy）是地方性肺炎的病原体，在集约化养猪生产系统中发病率高但死亡率低。为了更好地理解灭活全细胞疫苗的保护机制，我们研究了经实验感染Mhy的接种疫苗和未接种疫苗的猪细胞因子表达的免疫组化差异。将4周龄的Mhy阴性猪（n = 24）分为阴性对照组（n = 8），或两个感染Mhy的组之一：接种疫苗组（n = 8）和未接种疫苗组（n = 8）。通过气管内和雾化途径联合进行感染。使用针对Mhy、IL1-α、IL1-β、IL-2、IL-4、IL-5、IL-6、Il-8、IL-10、IL-12p35、IL-13、IL-17A、TNF-α、IFN-γ和CD-4淋巴细胞的抗体对肺样本进行组织病理学和免疫组化研究。尽管两个感染组中所有细胞因子均增加，但接种疫苗的猪中IL-2、IL-12和IFN-γ显著过表达。这些发现，结合接种疫苗动物宏观和组织学病变的减少，表明在控制Mhy感染的免疫策略中增强Th1反应的重要性。
