Hamel E, Assumel-Lurdin C, Edvinsson L, Fage D, MacKenzie E T
Department of Biology, LERS-Synthélabo, Bagneux, (France).
Brain Res. 1987 Sep 15;420(2):391-6. doi: 10.1016/0006-8993(87)91263-7.
Functional pial vessels denuded in situ of the endothelial cell layer exhibit a markedly decreased choline uptake capacity (-53%) but integrally preserved choline acetyltransferase (ChAT) activity and acetylcholine (ACh) release mechanisms. These studies demonstrate that endothelial cells possess a specific choline uptake system. However, the unimpaired ChAT activity in denuded pial vessels implies that the endothelial pool of choline is not significantly metabolized into ACh. In spite of possible differences in the mechanisms that govern release processes in endothelial and neuronal elements, taken together the findings of the present study suggest that the ACh released following depolarization of pial blood vessels originates predominantly from cholinergic perivascular nerve terminals.
原位剥除内皮细胞层的软脑膜功能性血管，其胆碱摄取能力显著降低（-53%），但胆碱乙酰转移酶（ChAT）活性和乙酰胆碱（ACh）释放机制整体保持完好。这些研究表明，内皮细胞拥有特定的胆碱摄取系统。然而，剥除内皮的软脑膜血管中ChAT活性未受影响，这意味着内皮细胞内的胆碱池不会大量代谢为ACh。尽管在内皮细胞和神经元成分中，调节释放过程的机制可能存在差异，但本研究结果总体表明，软脑膜血管去极化后释放的ACh主要源自胆碱能血管周围神经末梢。
