Tomlinson A, Van Vlijmen H, Loesch A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Cell Tissue Res. 1991 Jan;263(1):173-81. doi: 10.1007/BF00318413.
"En face" sheets of endothelium, in which cellular spatial relationships were maintained, were prepared from proximal pulmonary and femoral arteries and aortae of the Wistar rat, in order to visualise patterns of heterogeneity in populations of endothelial cells. These preparations, termed Häutchen, were immunolabelled with antibodies to angiotensin II, endothelin and Factor-VIII-related antigen, and visualised by an avidin/biotin peroxidase complex. Clusters of cells, which accounted for approximately 30% of the total endothelial cell population, and which were positively immunostained for angiotensin II, were found perpendicular to the longitudinal axis of the aorta and femoral artery. Cells in the pulmonary artery were immunonegative for angiotensin II. The majority of cells in all three vessels were immunopositive for endothelin; groups of intensely stained cells were present in both the femoral artery and aorta, but not in the pulmonary artery. Immunoreactivity to Factor-VIII-related antigen was heterogeneous, with intensely stained amorphous patches of endothelial cells present in the femoral artery and aorta. Häutchen preparations present an opportunity for the investigation of endothelial cell heterogeneity, both within and between vessels; this may provide a basis for the interpretation of the heterogeneity of endothelium-dependent responses in vessels of differing origin.
为了观察内皮细胞群体中的异质性模式，从Wistar大鼠的近端肺动脉、股动脉和主动脉制备了保持细胞空间关系的“正面”内皮片。这些制备物被称为“薄片”，用抗血管紧张素II、内皮素和因子VIII相关抗原的抗体进行免疫标记，并通过抗生物素蛋白/生物素过氧化物酶复合物进行观察。发现占内皮细胞总数约30%的细胞簇，它们对血管紧张素II呈阳性免疫染色，垂直于主动脉和股动脉的纵轴。肺动脉中的细胞对血管紧张素II呈免疫阴性。所有三种血管中的大多数细胞对内皮素呈免疫阳性；股动脉和主动脉中都有强染色细胞群，但肺动脉中没有。对因子VIII相关抗原的免疫反应性是异质性的，股动脉和主动脉中有强染色的无定形内皮细胞斑块。薄片制备物为研究血管内和血管间的内皮细胞异质性提供了机会；这可能为解释不同来源血管中内皮依赖性反应的异质性提供基础。
