Burnard D M, Veale W L, Pittman Q J
Department of Medical Physiology, Faculty of Medicine, University of Calgary, Alta., Canada.
Brain Res. 1987 Sep 29;422(1):11-6. doi: 10.1016/0006-8993(87)90534-8.
Extracellular recordings from cells in the CA1b region of the in vitro hippocampal slice preparation demonstrate that bath-applied AVP (10(-6)-10(-12) M) frequently results in a decrease in the orthodromically evoked population spike amplitude. This suggests that AVP inhibits CA1 pyramidal cell firing in response to an orthodromic volley. This effect appears to be receptor-mediated, since a potent antagonist of the AVP V1 (vasopressor) receptor and a mixed oxytocin/vasopressin antagonist prevented the decrease in population spike amplitude observed in response to bath application of AVP. Hippocampal slices prepared from rats injected two days earlier with 1.0 micrograms AVP (intracerebroventricular) display increased sensitivity to the depressant effects of AVP at lower doses compared to controls. These results suggest that pretreatment of rats with AVP may alter the sensitivity of hippocampal cells to the depressant effects of this neuropeptide.
对体外海马脑片制备中CA1b区细胞进行的细胞外记录表明，浴加精氨酸加压素（AVP，10⁻⁶ - 10⁻¹² M）常常会导致顺向诱发群体峰电位幅度降低。这表明AVP抑制CA1锥体细胞对顺向冲动的放电。这种效应似乎是由受体介导的，因为AVP V1（升压）受体的强效拮抗剂以及一种催产素/加压素混合拮抗剂可阻止在浴加AVP时观察到的群体峰电位幅度降低。与对照组相比，由两天前经脑室内注射1.0微克AVP的大鼠制备的海马脑片，在较低剂量下对AVP的抑制作用表现出更高的敏感性。这些结果表明，用AVP预处理大鼠可能会改变海马细胞对这种神经肽抑制作用的敏感性。
