Albeck D, Smock T
Department of Psychology, University of Colorado, Boulder 80309.
Brain Res. 1988 Nov 1;463(2):394-7. doi: 10.1016/0006-8993(88)90417-9.
The action of arginine vasopressin (AVP) in the rat hippocampal slice has been extensively studied. Extracellular recording indicates that the peptide excites spontaneously active neurons in the slice, though uncertainty exists regarding the identity of this cell type. Intracellular recording from pyramidal cells also reveals an excitatory action of the peptide, but these results are confounded by simultaneous constriction of small blood vessels that surround each pyramidal cell. Here we use field potential recordings to show that AVP inhibits pyramidal cell discharge and employs a pressor-type (V1) receptor to bring about its action. The results resolve issues regarding the identity of AVP targets in the slice. Each reported result is consistent with a model that posits direct AVP excitation of inhibitory interneurons and direct AVP constriction of slice microvessels. Inhibition of pyramidal cells recorded extracellularly and excitation of pyramidal cells recorded intracellularly are respective indirect consequences of the two direct effects.
精氨酸加压素（AVP）在大鼠海马切片中的作用已得到广泛研究。细胞外记录表明，该肽能激发切片中自发活动的神经元，不过关于这种细胞类型的身份存在不确定性。对锥体细胞进行的细胞内记录也揭示了该肽的兴奋作用，但这些结果因围绕每个锥体细胞的小血管同时收缩而受到混淆。在这里，我们使用场电位记录来表明AVP抑制锥体细胞放电，并利用一种升压型（V1）受体来实现其作用。这些结果解决了关于切片中AVP靶点身份的问题。每个报告的结果都与一个模型一致，该模型假定AVP直接兴奋抑制性中间神经元，并直接使切片微血管收缩。细胞外记录的锥体细胞抑制和细胞内记录的锥体细胞兴奋分别是这两种直接效应的间接结果。
