Earnest D J, Sladek C D
University of Rochester Medical Center, Department of Neurobiology/Anatomy, NY 14642.
Brain Res. 1987 Oct 6;422(2):398-402. doi: 10.1016/0006-8993(87)90952-8.
Using a perifusion culture technique, the pattern of vasopressin release from the suprachiasmatic nucleus was studied in vitro under basal and stimulated conditions. Individual rat suprachiasmatic explants released vasopressin in a rhythmic fashion and these rhythms persisted with a circadian period for up to 4 cycles. One-hour exposures to KCl on days 2 and 4 in culture induced acute increases in vasopressin output and altered the circadian pattern of release from perifused suprachiasmatic explants. These results provide evidence that the release of vasopressin in an acute as well as a circadian fashion from isolated suprachiasmatic neurons is indicative of active release processes. Furthermore, it is anticipated that this method of studying circadian rhythms generated by suprachiasmatic explants may provide a basis for elucidating the neural organization of mammalian circadian pacemakers.
利用灌流培养技术，在体外基础和刺激条件下研究了视交叉上核中血管加压素的释放模式。单独的大鼠视交叉上核外植体以节律性方式释放血管加压素，这些节律以昼夜周期持续长达4个周期。在培养的第2天和第4天，用氯化钾处理1小时可诱导血管加压素输出急性增加，并改变灌流视交叉上核外植体的昼夜释放模式。这些结果证明，分离的视交叉上核神经元以急性和昼夜节律方式释放血管加压素表明存在活跃的释放过程。此外，预计这种研究视交叉上核外植体产生的昼夜节律的方法可能为阐明哺乳动物昼夜节律起搏器的神经组织提供基础。
