来自大鼠视交叉上核的长期培养神经元保留了血管加压素释放的昼夜节律振荡能力。

Long-term cultured neurons from rat suprachiasmatic nucleus retain the capacity for circadian oscillation of vasopressin release.

作者信息

Murakami N, Takamure M, Takahashi K, Utunomiya K, Kuroda H, Etoh T

机构信息

Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Japan.

出版信息

Brain Res. 1991 Apr 5;545(1-2):347-50. doi: 10.1016/0006-8993(91)91312-o.

DOI:10.1016/0006-8993(91)91312-o

PMID:1860057

Abstract

We have developed a dissociated suprachiasmatic nucleus (SCN) cell culture system in order to begin a cellular analysis of the mammalian circadian oscillator. Monolayer-cultured neurons prepared from the SCN regions of 1-day-old rats were maintained in serum-free culture medium for 3-4 weeks and then perfused. In 12 out of 40 wells, a circadian oscillation of vasopressin release persisted for at least 4-5 cycles until the end of the perfusion period with no damping of the amplitude. This finding suggests that cultured SCN neurons retain the capacity for circadian oscillation for a long time, and should provide a useful model for the analysis of a mammalian circadian system at the cellular level.

摘要

为了对哺乳动物昼夜节律振荡器进行细胞分析，我们开发了一种分离的视交叉上核（SCN）细胞培养系统。从1日龄大鼠的SCN区域制备的单层培养神经元在无血清培养基中培养3 - 4周，然后进行灌注。在40个孔中的12个孔中，加压素释放的昼夜节律振荡持续至少4 - 5个周期，直到灌注期结束，振幅没有衰减。这一发现表明，培养的SCN神经元长时间保留昼夜节律振荡的能力，应为在细胞水平分析哺乳动物昼夜节律系统提供一个有用的模型。