Pavlides C, Aoki C, Chen J S, Bailey W H, Winson J
Rockefeller University, New York, NY 10021.
Brain Res. 1987 Oct 13;423(1-2):399-402. doi: 10.1016/0006-8993(87)90871-7.
Regional cerebral glucose utilization (CGU), detectable by the uptake of 2-deoxy-[14C]glucose [( 14C]2DG), was examined during 3 behavioral states--slow-wave sleep (SWS), the still-alert state (SAL) and locomotion (LOC). Examination of the autoradiograms, generated by exposing the [14C]2DG incorporated brain sections to Kodak Royal X Pan film revealed a high level of uptake bilaterally and discretely in the parafascicular (PF) region during these behaviors. This pattern of [14C]2DG uptake does not correspond to any of the anatomical structures previously identified by histo- and cytochemical methods, including the [14C]2DG method. Further, optical density measurements of this region indicated that the [14C]2DG uptake was significantly lower during SWS than during SAL or LOC. The present finding is compatible with the interpretations of previous physiological and behavioral studies that there is an inhibition by cells at the PF relay to the dentate gyrus that is lowered during the SWS compared to the SAL state, thus allowing preferential brain-stem activation of the dentate gyrus.
通过2-脱氧-[14C]葡萄糖（[14C]2DG）摄取可检测到的局部脑葡萄糖利用（CGU），在三种行为状态下进行了检测——慢波睡眠（SWS）、静息警觉状态（SAL）和运动（LOC）。将掺入[14C]2DG的脑切片暴露于柯达皇家X Pan胶片上生成放射自显影片，对其进行检查发现，在这些行为过程中，双侧束旁（PF）区域有高水平且离散的摄取。这种[14C]2DG摄取模式与先前通过组织学和细胞化学方法（包括[14C]2DG方法）鉴定的任何解剖结构均不对应。此外，该区域的光密度测量表明，SWS期间的[14C]2DG摄取明显低于SAL或LOC期间。目前的发现与先前的生理学和行为学研究解释一致，即PF中继至齿状回的细胞存在抑制作用，与SAL状态相比，SWS期间这种抑制作用降低，从而使得齿状回优先被脑干激活。
