Genome-Wide Analysis of ATP Binding Cassette (ABC) Transporters in Peach () and Identification of a Gene Involved in Anthocyanin Accumulation.

The ATP-binding cassette (ABC) transporter family is a large and diverse protein superfamily that plays various roles in plant growth and development. Although the ABC transporters are known to aid in the transport of a wide range of substrates across biological membranes, their role in anthocyanin transport remains elusive. In this study, we identified a total of 132 putative genes in the peach genome, and they were phylogenetically classified into eight subfamilies. Variations in spatial and temporal gene expression levels resulted in differential expression patterns of family members in various tissues of peach. was identified as the most likely candidate gene essential for anthocyanin accumulation in peach. Transient overexpression of caused a significant increase in anthocyanin accumulation in tobacco leaves and peach fruit, whereas virus-induced gene silencing of in the blood-fleshed peach resulted in a significant decrease in anthocyanin accumulation. The promoter contained an MYB binding -element, and it could be activated by anthocyanin-activator PpMYB10.1 based on yeast one-hybrid and dual luciferase assays. Thus, it seems that plays a crucial role in anthocyanin accumulation in peach. Our results provide a new insight into the vacuolar transport of anthocyanins in peach.