Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 121352 Moscow, Russia.
Cell Biophysics Lab., State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia.
Int J Mol Sci. 2023 Jan 22;24(3):2184. doi: 10.3390/ijms24032184.
Endometriosis of the cervix is a rare form of genital endometriosis, which is characterized by the appearance of tissue on the vaginal part of the cervix, similar to the tissue of the mucous membrane of the uterine cavity. We describe a clinical case in which we compared the content of cytoskeletal proteins, H3 histone modifications and DNA methylation (total and 5-hydroxymethylcytosine content) in the eutopic endometrium and in tissue from endometriosis foci on the cervix. The patient had elevated levels of estradiol, interleukin-1β and interleukin-8. At the cellular level, the content of tubulin and the marker of stable microtubules were reduced in the ectopic endometrium (by 45% and 37%, < 0.05, respectively), but the alpha-actinin-1 content was increased (by 75%, < 0.05) with an increase in the expression of its gene. At the same time, the total level of DNA methylation in the endometriotic focus was reduced by more than 2 times with the accumulation of the intermediate product 5-hydroxymethylcytosine (the content increased by more than 3 times), probably due to an increase in the content of tet methylcytosine dioxygenase 1 (more than 4 times).
宫颈子宫内膜异位症是一种罕见的生殖器子宫内膜异位症，其特征是在宫颈阴道部分出现类似于子宫腔粘膜组织的组织。我们描述了一个临床病例，比较了宫颈子宫内膜异位症病灶组织和在位子宫内膜中细胞骨架蛋白、H3 组蛋白修饰和 DNA 甲基化（总甲基化和 5-羟甲基胞嘧啶含量）的含量。患者的雌二醇、白细胞介素-1β 和白细胞介素-8 水平升高。在细胞水平上，异位子宫内膜中微管蛋白和稳定微管标记物的含量降低（分别降低 45%和 37%， < 0.05），但 α-辅肌动蛋白-1 的含量增加（增加 75%， < 0.05），其基因表达增加。同时，子宫内膜异位症病灶中的总 DNA 甲基化水平降低了两倍以上，中间产物 5-羟甲基胞嘧啶积累（含量增加了三倍以上），这可能是由于四甲基环化酶 1 的含量增加（增加了四倍以上）。
