两成分系统反应调节子 UvrY 在肠出血性 O157:H7 致病性调节中的新作用。

A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic O157:H7 Pathogenicity Regulation.

机构信息

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.

Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China.

出版信息

Int J Mol Sci. 2023 Jan 24;24(3):2297. doi: 10.3390/ijms24032297.

DOI:10.3390/ijms24032297

PMID:36768620

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9916836/

Abstract

Enterohemorrhagic (EHEC) O157:H7 is an important human pathogen causing severe diseases, such as hemorrhagic colitis and lethal hemolytic uremic syndrome. The signal-sensing capability of EHEC O157:H7 at specific host colonization sites via different two-component systems (TCSs) is closely related to its pathogenicity during infection. However, the types of systems involved and the regulatory mechanisms are not fully understood. Here, we investigated the function of the TCS BarA/UvrY regulator UvrY in the pathogenicity regulation of EHEC O157:H7. Our results showed that UvrY acts as a positive regulator of EHEC O157:H7 for cellular adherence and mouse colonization through the transcriptional activation of the locus for enterocyte effacement (LEE) pathogenic genes. Furthermore, this regulation is mediated by the LEE island master regulator, Ler. Our results highlight the significance of UvrY in EHEC O157:H7 pathogenicity and underline the unknown importance of BarA/UvrY in colonization establishment and intestinal adaptability during infection.

摘要

肠出血性大肠杆菌（EHEC）O157：H7 是一种重要的人类病原体，可引起严重疾病，如出血性结肠炎和致命性溶血性尿毒综合征。EHEC O157：H7 通过不同的双组分系统（TCS）在特定宿主定植部位的信号感应能力与其感染过程中的致病性密切相关。然而，涉及的系统类型和调节机制尚不完全清楚。在这里，我们研究了 TCS BarA/UvrY 调节剂 UvrY 在 EHEC O157：H7 致病性调节中的作用。我们的结果表明，UvrY 通过转录激活肠上皮细胞脱落（LEE）致病基因，作为 EHEC O157：H7 细胞黏附和小鼠定植的正调节剂。此外，这种调节是由 LEE 岛主调节因子 Ler 介导的。我们的结果强调了 UvrY 在 EHEC O157：H7 致病性中的重要性，并强调了 BarA/UvrY 在感染过程中定植建立和肠道适应性方面的未知重要性。