Unité Transfrontalière BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), UMRt 1158, Université de Picardie Jules Verne, 80039 Amiens, France.
Plateforme d'Ingénierie Cellulaire & Analyses des Protéines ICAP, FR CNRS 3085 ICP, Université de Picardie Jules Verne, 80039 Amiens, France.
Int J Mol Sci. 2023 Jan 25;24(3):2390. doi: 10.3390/ijms24032390.
The rapeseed crop is susceptible to many pathogens such as parasitic plants or fungi attacking aerial or root parts. Conventional plant protection products, used intensively in agriculture, have a negative impact on the environment as well as on human health. There is therefore a growing demand for the development of more planet-friendly alternative protection methods such as biocontrol compounds. Natural rhamnolipids (RLs) can be used as elicitors of plant defense mechanisms. These glycolipids, from bacteria secretome, are biodegradable, non-toxic and are known for their stimulating and protective effects, in particular on rapeseed against filamentous fungi. Characterizing the organ responsiveness to defense-stimulating compounds such as RLs is missing. This analysis is crucial in the frame of optimizing the effectiveness of RLs against various diseases. A Tandem Mass Tags (TMT) labeling of the proteins extracted from the shoots and roots of rapeseed has been performed and showed a differential pattern of protein abundance between them. Quantitative proteomic analysis highlighted the differential accumulation of parietal and cytoplasmic defense or stress proteins in response to RL treatments with a clear effect of the type of application (foliar spraying or root absorption). These results must be considered for further use of RLs to fight specific rapeseed pathogens.
油菜作物容易受到许多病原体的侵袭，如寄生植物或真菌攻击地上或地下部分。在农业中广泛使用的传统植保产品对环境和人类健康都有负面影响。因此，人们越来越需要开发更环保的替代保护方法，如生物防治化合物。天然鼠李糖脂（RL）可用作植物防御机制的激发剂。这些从细菌分泌物中提取的糖脂可生物降解，无毒，具有刺激和保护作用，特别是对油菜抵抗丝状真菌。目前还缺乏对 RL 等具有防御刺激作用的化合物的器官反应性的特征描述。在优化 RL 对各种疾病的有效性方面，这一分析至关重要。对油菜地上部和根部提取的蛋白质进行了串联质量标签（TMT）标记，结果显示它们之间的蛋白质丰度存在差异模式。定量蛋白质组学分析突出了 RL 处理后质膜和细胞质防御或应激蛋白的差异积累，且应用方式（叶面喷施或根部吸收）有明显的影响。在进一步使用 RL 防治特定油菜病原菌时，必须考虑这些结果。
