Agronomy College, Guizhou University, Huaxi, Guiyang 550025, China.
Int J Mol Sci. 2023 Feb 1;24(3):2765. doi: 10.3390/ijms24032765.
The regulation of microsomal (e.g., FAD2) and plastidial (e.g., FAD6) oleate desaturases by cold, heat and salt stress were investigated. Gene expression levels and fatty acid compositions were determined in the roots, stems and leaves of safflower following stress treatments. A safflower plastidial oleate desaturase gene, , was cloned, and oleic acid desaturation was confirmed in sp. strain PCC7942. The results showed that temperature regulated oleate desaturation at the transcriptional level, and this regulation pattern was tissue-specific. , and were significantly induced under cold and heat stress in young leaves, and and were slightly induced in young stems. In contrast, , and were sensitive to salt stress in all safflower tissues (roots, stem and leaves). was insensitive to salt and was slightly induced in leaves only.
研究了微体（如 FAD2）和质体（如 FAD6）油酸去饱和酶在冷、热和盐胁迫下的调节。在胁迫处理后，测定了红花根、茎和叶中的基因表达水平和脂肪酸组成。克隆了红花质体油酸去饱和酶基因 ，并在 sp. PCC7942 菌株中证实了油酸的去饱和作用。结果表明，温度在转录水平上调节油酸去饱和作用，这种调节模式具有组织特异性。在幼叶中， 、 和 在冷、热胁迫下显著诱导，而 、 在幼茎中略有诱导。相比之下， 、 和 在红花所有组织（根、茎和叶）中对盐胁迫敏感。 对盐不敏感，仅在叶片中略有诱导。
