Hernández M Luisa, Padilla María N, Mancha Manuel, Martínez-Rivas José M
Instituto de la Grasa (CSIC), 41012 Sevilla, Spain.
J Agric Food Chem. 2009 Jul 22;57(14):6199-206. doi: 10.1021/jf900678z.
The effect of ripening stage and water regimen on oleate desaturase gene expression levels in the fruit of different olive ( Olea europaea L.) varieties was investigated to elucidate the contribution of each to the linoleic acid content in virgin olive oil. To this end, fatty acid analysis and quantitative real time PCR were performed using distinct olive tissues and different developmental stages from the Picual and Arbequina cultivars. The results showed that the olive FAD2-1, FAD2-2, and FAD6 genes were spatial and temporally regulated. In addition, the data indicated that FAD2-2 seems to be the main gene responsible for the linoleic acid content in the olive fruit mesocarp tissue. This conclusion was also confirmed when the study was extended to Hojiblanca, Picudo, and Manzanilla varieties. With regard to the water regimen, unlike the Picual cultivar, a small increase of linoleic acid was observed in the Arbequina variety cultivated with irrigation, which correlated well with the increase detected for the FAD2-2 gene expression level. All of these data strongly suggest that FAD2-2 is the main gene that determines the linoleic acid content in the virgin olive oil.
研究了成熟阶段和水分管理对不同橄榄（油橄榄）品种果实中油酸去饱和酶基因表达水平的影响，以阐明它们各自对初榨橄榄油中亚油酸含量的贡献。为此，使用皮夸尔（Picual）和阿贝奎纳（Arbequina）品种不同的橄榄组织和不同发育阶段进行了脂肪酸分析和定量实时PCR。结果表明，橄榄FAD2-1、FAD2-2和FAD6基因受到时空调控。此外，数据表明FAD2-2似乎是负责橄榄果实中果皮组织中亚油酸含量的主要基因。当该研究扩展到霍吉布兰卡（Hojiblanca）、皮库多（Picudo）和曼萨尼亚（Manzanilla）品种时，这一结论也得到了证实。关于水分管理，与皮夸尔品种不同，在灌溉栽培的阿贝奎纳品种中观察到亚油酸略有增加，这与FAD2-2基因表达水平的增加密切相关。所有这些数据都有力地表明，FAD2-2是决定初榨橄榄油中亚油酸含量的主要基因。
