Yang Huanling, Jiang Jun, Chen Mingjie, Song Xiaoxia, Yu Changxia, Chen Hongyu, Zhao Yan
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, China.
J Fungi (Basel). 2024 Jul 18;10(7):496. doi: 10.3390/jof10070496.
Delta-12 fatty acid desaturases (FAD2s) actively regulate stress responses and cell differentiation in living organisms. In this study, six homologous genes were identified based on the genome sequence of . Then, the six FAD2 protein sequences were analyzed using bioinformatics tools, including ExPASy ProtParam, SignalP, TMHMM, and TargetP. These analyses were performed to predict the physical and chemical properties, signal peptides, and transmembrane and conserved domains of these proteins. The polypeptide sequences were aligned, and a maximum likelihood phylogenetic tree was constructed using MEGA 7.0 software to elucidate the phylogenetic relationships between homologous sequences. The results demonstrated that the FAD2 proteins contained three conserved histidine-rich regions (HXXXH, HXXHH, and HXXHH), which included eight histidine residues. The linoleic acid content and FAD2 enzyme activity were further analyzed, and the levels in the mutagenic heat-tolerant strain 18N44 were lower than those in the wild-type strain 18. Interestingly, the expression levels of the and genes under heat stress in strain 18N44 were lower than those in strain 18. These findings indicated that and may play major roles in the synthesis of linoleic acid during heat stress.
Δ-12脂肪酸去饱和酶（FAD2s）积极调控生物体内的应激反应和细胞分化。在本研究中，基于[具体物种名称]的基因组序列鉴定出六个同源基因。然后，使用包括ExPASy ProtParam、SignalP、TMHMM和TargetP在内的生物信息学工具对这六个FAD2蛋白序列进行分析。进行这些分析是为了预测这些蛋白质的物理和化学性质、信号肽、跨膜结构域和保守结构域。对多肽序列进行比对，并使用MEGA 7.0软件构建最大似然系统发育树，以阐明同源序列之间的系统发育关系。结果表明，FAD2蛋白包含三个保守的富含组氨酸区域（HXXXH、HXXHH和HXXHH），其中包括八个组氨酸残基。进一步分析了亚油酸含量和FAD2酶活性，诱变耐热菌株18N44中的水平低于野生型菌株18中的水平。有趣的是，菌株18N44在热胁迫下[具体基因名称1]和[具体基因名称2]的表达水平低于菌株18中的表达水平。这些发现表明，[具体基因名称1]和[具体基因名称2]可能在热胁迫期间亚油酸的合成中起主要作用。
