Laboratory of Molecular Oncology, Department of Thoracic Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zürich, Switzerland.
Functional Genomics Center Zurich, ETH Zurich and University of Zurich, 8057 Zurich, Switzerland.
Int J Mol Sci. 2023 Feb 2;24(3):2874. doi: 10.3390/ijms24032874.
Pleural mesothelioma (PM) is a cancer where epithelioid, biphasic and sarcomatoid histotypes are observed. Sarcomatoid PM is characterized by mesenchymal features. Multi-omics have been used to characterize the epithelial-to-mesenchymal (EMT) phenotype at the molecular level. We contribute to this effort by including the analysis of RNA editing. We extracted samples with the highest vs. lowest Epithelial score from two PM cohorts and observed increased RNA editing in introns and decreased RNA editing in 3'UTR upon EMT. The same was observed in primary PM primary cultures stratified by transcriptomics analysis into two groups, one of them enriched with mesenchymal features. Our data demonstrate that, as has been observed in other cancer types, RNA editing associates to EMT phenotype in PM.
胸膜间皮瘤 (PM) 是一种观察到上皮样、双相和肉瘤样组织型的癌症。肉瘤样 PM 的特征是间充质特征。多组学已被用于在分子水平上表征上皮-间充质 (EMT) 表型。我们通过包括 RNA 编辑分析来为此项工作做出贡献。我们从两个 PM 队列中提取具有最高与最低上皮评分的样本，并观察到 EMT 时内含子中的 RNA 编辑增加，3'UTR 中的 RNA 编辑减少。在通过转录组分析分层为两个组的原发性 PM 原代培养物中也观察到了同样的情况，其中一个组富含间充质特征。我们的数据表明，正如在其他癌症类型中观察到的那样，RNA 编辑与 PM 中的 EMT 表型相关。
