Laboratory of Useful Materials, National Institute of Research and Pysico-Chemical Analysis (INRAP), Technopark of Sidi Thabet, Ariana 2020, Tunisia.
Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Bizerte 7021, Tunisia.
Molecules. 2023 Jan 20;28(3):1073. doi: 10.3390/molecules28031073.
In this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert's experimental design applied the determination of the optimal operating conditions. An investigation into the effects of the methanol composition (50-100%), the volume of eluent (1-12 mL), and pH (1-3) on the extraction of phenols acids and total phenols from Tunisian olive oils was performed. The results showed that the extraction conditions had a significant effect on the extraction efficiency. The experiment showed that the greatest conditions for the SPE of phenolic acids were the methanol composition at 90.3%, pH at 2.9, and volume at 7.5 mL, respectively. The optimal conditions were applied to different types of olive oils, and it could be concluded that larger concentrations of polyphenols were found in extra virgin olive oil (89.15-218), whereas the lowest levels of these compounds (66.8 and 5.1) were found in cold-pressed crude olive oil and olive pomace oil, respectively.
在这项工作中，建立了一种使用新型亲水相互作用固相萃取（SPE）快速、精确且具有成本效益的方法来定量橄榄油中的酚类化合物。Boehlert 的实验设计用于确定最佳操作条件。研究了甲醇组成（50-100%）、洗脱液体积（1-12 mL）和 pH 值（1-3）对从突尼斯橄榄油中提取酚酸和总酚的影响。结果表明，提取条件对提取效率有显著影响。实验表明，酚酸 SPE 的最佳条件分别为甲醇组成 90.3%、pH 值 2.9 和体积 7.5 mL。最佳条件应用于不同类型的橄榄油，结果表明特级初榨橄榄油（89.15-218）中多酚浓度较高，而冷榨粗橄榄油和橄榄渣油中这些化合物的含量最低（分别为 66.8 和 5.1）。
