Zhu Jing, Zhang Ling, Ji Mengxia, Jin Bihui, Shu Jing
Department of Reproductive Endocrinology, Reproductive Medicine Center, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China.
J Obstet Gynaecol Res. 2023 Apr;49(4):1173-1179. doi: 10.1111/jog.15565. Epub 2023 Feb 11.
Redistribution of adipose tissue in the abdomen during the menopausal transition is attributable mostly to estrogen drop with aging. Adipose differentiation-related protein (ADRP), a major component of lipid droplets, is closely related to the onset of lipid accumulation. We hypothesized that estrogen exerted its tissue-specific effect in reducing abdominal fat accumulation by regulation of ADRP.
Twenty-four female C57/BL6 mice aged 8 weeks were randomly divided into 3 groups: sham operation (Sham), bilateral ovariectomy (OVX), and OVX plus 17β-estradiol (OVX + E2). After being fed 8 weeks of a high-fat diet, plasma lipid profiles including total cholesterol (TC), total triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels, body weight gain, visceral, and subcutaneous adipose tissue, adipocyte size, and ADRP expression were measured.
In comparison to sham-operated mice, OVX mice presented a weight gain with higher plasma TC, TG, LDL-C levels, and lower HDL-C levels. E2 supplement ameliorated the increase in weight and lipid profiles. Elevated ADRP expression was observed in visceral adipose tissue of OVX mice, whereas treatment of estrogen suppressed the ADPR expression and reversed the fat accumulation in the abdomen. However, no significant difference of ADRP expression in subcutaneous adipose tissue was detected between sham, OVX, and OVX + E2 mice.
Our findings suggested that enhanced ADRP expression in ovariectomized mice correlates with the tissue-specific regulation of estrogen, which may provide useful clues for further exploring the regulatory mechanism and corresponding anti-abdominal obesity treatment in postmenopausal women.
绝经过渡期腹部脂肪组织的重新分布主要归因于随着年龄增长雌激素水平下降。脂肪分化相关蛋白（ADRP）是脂滴的主要成分，与脂质积累的发生密切相关。我们推测雌激素通过调节ADRP发挥其减少腹部脂肪堆积的组织特异性作用。
将24只8周龄的雌性C57/BL6小鼠随机分为3组：假手术组（Sham）、双侧卵巢切除术组（OVX）和卵巢切除加17β-雌二醇组（OVX + E2）。在给予高脂饮食8周后，测量血浆脂质谱，包括总胆固醇（TC）、总甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平、体重增加、内脏和皮下脂肪组织、脂肪细胞大小以及ADRP表达。
与假手术小鼠相比，OVX小鼠体重增加，血浆TC、TG、LDL-C水平升高，HDL-C水平降低。补充E2改善了体重和脂质谱的增加。在OVX小鼠的内脏脂肪组织中观察到ADRP表达升高，而雌激素治疗抑制了ADPR表达并逆转了腹部脂肪堆积。然而，在假手术、OVX和OVX + E2小鼠的皮下脂肪组织中，未检测到ADRP表达的显著差异。
我们的研究结果表明，去卵巢小鼠中ADRP表达增强与雌激素的组织特异性调节相关，这可能为进一步探索绝经后妇女的调节机制和相应的抗腹部肥胖治疗提供有用线索。
