Plant Proteomic Research Center, Fujian Agriculture and Forestry University, Fuzhou, China.
Methods Mol Biol. 2023;2604:317-325. doi: 10.1007/978-1-0716-2867-6_26.
Many studies from different model organisms have demonstrated that microtubules are essential for various cellular processes, including cell division, cell morphogenesis, and intracellular trafficking. In interphase plant cells, oriented cortical microtubule arrays are highly characteristic in cells that display various morphologies, such as elongated hypocotyl cells and root cells, jigsaw-puzzled leaf pavement cells, and petal epidermal conical cells. Conical cells represent a specialized epidermal cell type found in the petal epidermis of many flowering plants. It has been suggested that in the model plant Arabidopsis thaliana, the petal adaxial epidermal cells develop from a roughly hemispherical morphology to a conical shape, correlating with the reorientation of cortical microtubules from random to well-ordered circumferential arrays. This chapter presents an overview of the methods available to visualize the microtubule cytoskeleton in living conical cells via confocal microscopy.
许多来自不同模式生物的研究表明，微管对于各种细胞过程都是必不可少的，包括细胞分裂、细胞形态发生和细胞内运输。在间期植物细胞中，定向的皮质微管阵列在表现出各种形态的细胞中高度特征化，例如伸长的下胚轴细胞和根细胞、拼图般的叶表皮铺展细胞和花瓣表皮锥形细胞。锥形细胞代表了在许多开花植物的花瓣表皮中发现的一种特殊表皮细胞类型。有人提出，在模式植物拟南芥中，花瓣腹侧表皮细胞从大致半球形形态发育成锥形，与皮质微管从随机到有序的周向排列的重新定向相关。本章介绍了通过共聚焦显微镜可视化活锥形细胞中微管细胞骨架的可用方法概述。
