Wang Weidong, Mei Lijuan, Yue Huilan, Tao Yanduo, Liu Zenggen
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, China.
Qinghai Provincial Key Laboratory of Tibetan Medicine Research and Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, CAS, Xining, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Feb 15;1217:123620. doi: 10.1016/j.jchromb.2023.123620. Epub 2023 Jan 27.
Saussurea obvallata (S. obvallata) is widely used in Qinghai-Tibet Plateau with high medicinal and edible values of reducing inflammation. But, the individual components and mechanisms of action still ill-defined. In this work, an integrated method using affinity ultrafiltration combined with preparative liquid chromatography was developed to identify and separate cyclooxygenase-2 (COX-2) inhibitors from S. obvallata. The sample was pretreated using on-line medium pressure liquid chromatography to yield the active fraction. Then, the potential COX-2 ligands were screened out using affinity ultrafiltration from the targeted fraction and the identified compounds were isolated via preparative liquid chromatography. As a result, four main compounds, coniferin (1), syringin (2), roseoside (3) and grasshopper ketone (4) were targeted isolated with IC values of 12.34 ± 1.81, 4.04 ± 0.43, 13.91 ± 2.46 and 7.97 ± 1.21 µM, respectively. Results of this work demonstrated that the developed strategy was effective for the targeted separation of COX-2 inhibitors from natural product extracts.
绵头雪兔子在青藏高原广泛使用，具有很高的抗炎药用和食用价值。但是，其具体成分和作用机制仍不明确。在本研究中，开发了一种亲和超滤结合制备液相色谱的综合方法，用于从绵头雪兔子中鉴定和分离环氧合酶-2（COX-2）抑制剂。样品通过在线中压液相色谱进行预处理，得到活性组分。然后，通过亲和超滤从目标组分中筛选出潜在的COX-2配体，并通过制备液相色谱分离鉴定出的化合物。结果，靶向分离出了四种主要化合物，松柏苷（1）、紫丁香苷（2）、蔷薇糖苷（3）和蚱蜢酮（4），其IC值分别为12.34±1.81、4.04±0.43、13.91±2.46和7.97±1.21μM。本研究结果表明，所开发的策略对于从天然产物提取物中靶向分离COX-2抑制剂是有效的。
