Zhang Shengnan, Wang Sujuan, Wang Shiyan, Su Hao, De Ji
Key Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, Lhasa 850000, China.
School of Ecology and Environment, Xizang University, Lhasa 850000, China.
Genes (Basel). 2025 May 17;16(5):593. doi: 10.3390/genes16050593.
BACKGROUND/OBJECTIVES: (DC.) Edgew., Asteraceae, is a traditional medicinal herbnative to the Qinghai-Tibet Plateau (QTP). Pharmacological investigationshave validated its pharmacological effects in anti-tumor, anti-inflammatory, heat-clearing, detoxifying, and analgesia. is presently facing habitat fragmentation and population decline. Therefore, we analyzed its genetic and chemical diversity to provide a scientific basis for the conservation and sustainable use of .
Seven populations of were sampled from Xizang, China. The genetic diversity was analyzed using inter-simple sequence repeat (ISSR) markers, and metabolites were identified by ultra-high-performance liquid chromatography-tandem-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS). Correlation analysis among genetic diversity, differential metabolites, and climatic factors were performed by R.
The genetic diversity among and within populations were both lowly and significantly correlated with geographical distance, showing a decreasing trend from east to west of the QTP. A total of 110 compounds were identified, including flavonoids, phenylpropanoids, lipids, fatty acids, terpenoids, alkaloids, etc. The metabolite contents among populations varied greatly and were related to environmental factors, mainly annual mean temperature and temperature fluctuation. The genetic diversity had little effect on the metabolic differences.
These findings provided valuable baseline information for the conservation and pharmacological utilization of . Meanwhile, further research is necessary for the efficacy evaluation of anti-inflammatory, anti-tumor, radiation protection, and scar removal both in vitro and in vivo.
背景/目的：菊科风毛菊属植物水母雪兔子是青藏高原特有的传统药用植物。药理研究证实了其在抗肿瘤、抗炎、清热、解毒和镇痛方面的药理作用。目前该植物正面临生境破碎化和种群数量下降的问题。因此，我们分析了其遗传和化学多样性，为水母雪兔子的保护和可持续利用提供科学依据。
从中国西藏采集了7个水母雪兔子种群的样本。利用简单重复序列区间（ISSR）标记分析遗传多样性，通过超高效液相色谱-串联四极杆-飞行时间质谱（UPLC-Q-TOF-MS/MS）鉴定代谢产物。利用R软件对遗传多样性、差异代谢产物和气候因子进行相关性分析。
种群间和种群内的遗传多样性均较低，且与地理距离显著相关，呈现出从青藏高原东部向西部递减的趋势。共鉴定出110种化合物，包括黄酮类、苯丙素类、脂质、脂肪酸、萜类、生物碱等。种群间代谢产物含量差异较大，且与环境因子有关，主要是年平均温度和温度波动。遗传多样性对代谢差异影响较小。
这些发现为水母雪兔子的保护和药理利用提供了有价值的基础信息。同时，有必要进一步开展体内外抗炎、抗肿瘤、辐射防护和祛疤功效评价的研究。
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