肥胖与类风湿关节炎患者中Dialister菌属的扩张有关。

Adiposity is associated with expansion of the genus Dialister in rheumatoid arthritis patients.

作者信息

Mena-Vázquez Natalia, Ruiz-Limón Patricia, Moreno-Indias Isabel, Manrique-Arija Sara, Lisbona-Montañez Jose Manuel, Rioja José, Mucientes Arkaitz, Martin-Núñez Gracia María, Cano-García Laura, Tinahones Francisco J, Fernández-Nebro Antonio

机构信息

The Biomedical Research Institute of Malaga and Platform in Nanomedicine (IBIMA BIONAND Platform), 29590 Málaga, Spain; UGC de Reumatología, Hospital Regional Universitario de Málaga, 29009 Málaga, Spain.

The Biomedical Research Institute of Malaga and Platform in Nanomedicine (IBIMA BIONAND Platform), 29590 Málaga, Spain; Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Clínico Virgen de la Victoria, 29010 Málaga, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain.

出版信息

Biomed Pharmacother. 2023 Apr;160:114388. doi: 10.1016/j.biopha.2023.114388. Epub 2023 Feb 9.

DOI:10.1016/j.biopha.2023.114388

PMID:36773522

Abstract

OBJECTIVE

To analyze the intestinal microbiota of patients with rheumatoid arthritis (RA) and obesity and a higher percentage of fatty tissue.

METHODS

Nested case-control study of 80 RA patients and 80 age and sex-matched controls. Obesity was defined as a body mass index ≥ 30, and body composition using dual-energy x-ray absorptiometry. The gut microbiota was analyzed using 16 S rRNA gene sequencing; bioinformatics analysis was performed using QIIME2 and PICRUSt. Other variables included averaged 28-joint Disease Activity Score (DAS28-ESR), cytokines and adipokines. Two multivariate were constructed with obesity and fat mass index (FMI).

RESULTS

Obesity was more frequent in RA patients than in controls (36.3 % vs 25.1 %; p = 0.026), as was a higher FMI value (mean [SE]=11.6 [3.9] vs 10.2 [3.9]; p = 0.032). Alpha and beta diversity analysis revealed differences in gut microbiota between RA patients with and without obesity. Dialister and Odoribacter were more abundant in RA patients with obesity than in RA patients without obesity, while the genus Clostridium was more abundant in RA patients without obesity. The factors associated with obesity in RA patients were age (OR [95 % CI], 1.09 [1.02-1.17]), mean DAS28-ESR (OR [95 % CI], 1.46 [1.12-1.67]), leptin levels (OR [95 % CI], 1.06 [1.01-1.10]), the genus Dialister (OR [95 % CI], 1.03 [1.01-1.07]), and the genus Clostridium (OR [95 % CI], 0.013 [0.00-0.36]). The associations observed for FMI were similar.

CONCLUSIONS

In patients with RA, obesity, and a higher percentage of fatty tissue, intestinal microbiota differed from that of controls and of the other patients. The genus Dialister was associated with obesity and FMI.

摘要

目的

分析类风湿关节炎（RA）合并肥胖及脂肪组织比例较高患者的肠道微生物群。

方法

对80例RA患者和80例年龄及性别匹配的对照进行巢式病例对照研究。肥胖定义为体重指数≥30，并采用双能X线吸收法测量身体成分。使用16S rRNA基因测序分析肠道微生物群；使用QIIME2和PICRUSt进行生物信息学分析。其他变量包括平均28个关节疾病活动评分（DAS28-ESR）、细胞因子和脂肪因子。构建了两个包含肥胖和脂肪质量指数（FMI）的多变量模型。

结果

RA患者中肥胖的发生率高于对照组（36.3%对25.1%；p = 0.026），FMI值也更高（平均值[标准误]=11.6[3.9]对10.2[3.9]；p = 0.032）。α和β多样性分析显示，肥胖和非肥胖RA患者的肠道微生物群存在差异。在肥胖的RA患者中，Dialister菌属和Odoribacter菌属比非肥胖的RA患者更为丰富，而梭菌属在非肥胖的RA患者中更为丰富。与RA患者肥胖相关的因素包括年龄（比值比[95%置信区间]，1.09[1.02 - 1.17]）、平均DAS28-ESR（比值比[95%置信区间]，1.46[1.12 - 1.67]）、瘦素水平（比值比[95%置信区间]，1.06[1.01 - 1.10]）、Dialister菌属（比值比[95%置信区间]，1.03[1.01 - 1.07]）和梭菌属（比值比[95%置信区间]，0.013[0.00 - 0.36]）。观察到的FMI相关关联相似。