科林斯氏菌与既定类风湿关节炎队列中的累积炎症负担有关。

Collinsella is associated with cumulative inflammatory burden in an established rheumatoid arthritis cohort.

机构信息

Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND, 29010 Málaga, Spain; Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Clínico Virgen de la Victoria, 29010 Málaga, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain.

Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND, 29010 Málaga, Spain; UGC de Reumatología, Hospital Regional Universitario de Málaga, 29009 Málaga, Spain.

出版信息

Biomed Pharmacother. 2022 Sep;153:113518. doi: 10.1016/j.biopha.2022.113518. Epub 2022 Aug 6.

DOI:10.1016/j.biopha.2022.113518

PMID:36076603

Abstract

OBJECTIVE

To analyze the gut microbiota of patients with rheumatoid arthritis (RA) according to disease activity.

METHODS

An observational cross-sectional study of 110 patients with RA and 110 age- and sex-matched controls was performed. Patients were classified according to the disease activity (DAS28 ≥3.2 or DAS28 <3.2). Clinical and epidemiological variables were included. The gut microbiota was analyzed using 16S rRNA sequencing and bioinformatics analysis based on QIIME and PICRUSt. A multivariate analysis was performed to identify factors associated with inflammatory activity.

RESULTS

The mean DAS28 indicated remission/low inflammatory activity in 71 patients (64.5 %) and moderate/high activity in 39 (35.5 %) during follow-up. Alpha and beta diversity analysis revealed differences in gut microbiota between the 3 study groups. In the moderate/high activity RA, we observed a significant change in the abundance of genera compared with the other groups. The abundance of Collinsella and Bifidobacterium was increased in RA patients compared with controls. The metabolic profile of gut microbiota was characterized by differences in pathways related to Biosynthesis, Generation of Precursor Metabolites/Energy, and Degradation/Utilization/Assimilation between the 3 groups. The factors associated with cumulative inflammatory activity in RA were age (OR [95 % CI], 1.065 [1.002-1.131]), obesity (OR [95% CI], 3.829 [1.064-8.785]), HAQ score (OR [95% CI], 2.729 [1.240-5.009]), and expansion of the genus Collinsella (OR [95% CI], 3.000 [1.754-9.940]).

CONCLUSIONS

The composition of gut microbiota differed between patients with RA and moderate/high activity, patients with remission/low activity, and controls. The genus Collinsella, age, obesity, and physical function were associated with cumulative inflammatory burden in RA.

摘要

目的

根据疾病活动度分析类风湿关节炎（RA）患者的肠道微生物群。

方法

对 110 例 RA 患者和 110 例年龄和性别匹配的对照进行了一项观察性横断面研究。根据疾病活动度（DAS28≥3.2 或 DAS28<3.2）对患者进行分类。纳入临床和流行病学变量。使用 16S rRNA 测序和基于 QIIME 和 PICRUSt 的生物信息学分析对肠道微生物群进行分析。采用多元分析确定与炎症活动相关的因素。

结果

在随访期间，71 例患者（64.5%）的平均 DAS28 提示缓解/低炎症活动，39 例（35.5%）为中度/高活动。α 和β多样性分析显示三组研究对象的肠道微生物群存在差异。在中度/高活动 RA 中，与其他两组相比，观察到属丰度的显著变化。与对照组相比，RA 患者的柯林斯氏菌和双歧杆菌丰度增加。肠道微生物群的代谢谱特征是三组之间与生物合成、前体代谢物/能量生成、降解/利用/同化相关的途径存在差异。与 RA 累积炎症活动相关的因素是年龄（OR[95%CI]，1.065[1.002-1.131]）、肥胖（OR[95%CI]，3.829[1.064-8.785]）、HAQ 评分（OR[95%CI]，2.729[1.240-5.009]）和柯林斯氏菌属的扩张（OR[95%CI]，3.000[1.754-9.940]）。