Testen Anna L, Shaw R Scott, Rotondo Francesca, Moodispaw Margaret R, Miller Sally A
USDA-ARS Application Technology Research Unit, Wooster, OH.
The Ohio State University Department of Plant Pathology, Wooster, OH.
Plant Dis. 2023 Sep;107(9):2673-2678. doi: 10.1094/PDIS-08-22-2009-RE. Epub 2023 Sep 13.
Corky root rot is an important disease in tomato production systems and is caused by and (formerly Types 1 and 2, respectively). The corky root rot pathogens are slow growing and difficult to isolate and quantify in soil and plant tissue. A multiplex hydrolysis probe-based qPCR assay was designed to allow for simultaneous detection and quantification of and with a competitive internal control to indicate if qPCR inhibitors are present. Single species and multiplex assays for spp. detected DNA levels above 0.013 pg of DNA per reaction. These highly specific assays had no nontarget amplification of other fungal and oomycete pathogens or rhizosphere-associated fungi of tomatoes that were tested. This assay can be used to quantify populations in roots and soils in tomato production systems to better determine the impacts of disease management strategies on spp. and provides a tool to study the biology of spp.
栓皮根腐病是番茄生产系统中的一种重要病害，分别由和（以前分别为1型和2型）引起。栓皮根腐病病原体生长缓慢，难以在土壤和植物组织中分离和定量。设计了一种基于多重水解探针的qPCR检测方法，用于同时检测和定量以及使用竞争性内对照来指示是否存在qPCR抑制剂。针对 spp. 的单物种和多重检测检测到每个反应中DNA水平高于0.013 pg DNA。这些高度特异性的检测方法对所测试的其他真菌和卵菌病原体或番茄根际相关真菌没有非靶标扩增。该检测方法可用于量化番茄生产系统中根和土壤中的种群，以更好地确定病害管理策略对 spp. 的影响，并提供一种研究 spp. 生物学的工具。
