ATAPA1 基因外显子 9 上 SNP 对鹅产蛋性能的作用。

Role of SNPs located in the exon 9 of ATAPA1 gene on goose egg production.

机构信息

Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R. China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, P. R. China.

出版信息

Poult Sci. 2023 Apr;102(4):102488. doi: 10.1016/j.psj.2023.102488. Epub 2023 Jan 11.

DOI:10.1016/j.psj.2023.102488

PMID:36774712

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9943896/

Abstract

The meat and egg of goose is one of the main components of human food supply. The improvement of goose egg production is particularly important for the increasing human population. However, limited information is available about the effective molecular markers and mechanisms of egg production in goose. In this study, we jointly utilized the data of genome resequencing in different egg production Sichuan white goose and transcriptome at different follicle development stages to identified the molecular markers and mechanisms of egg production. The coefficient of variation of individual egg production in Sichuan white goose population is 0.42 to 0.49. Fifty individuals with the highest (laying 365 days egg number, LEN365 = 79-145) and 50 individuals with the lowest (LEN365 = 8-48) egg production were divided into high and low egg production groups. Based on whole-genome sequencing data of the selected samples, 36 SNPs (annotation novel.12.470, CELF2, ATP1A1, KCNJ6, RAB4A, UST, REV3L, DHX15, CAVN2, SLC5A9, Cldn5, MRPS23, and Tspan2) associated with the LEN365 were identified, involving multiple pathways such as metabolism and endocrinology. Notably, 5 SNPs located in the exon9 of ATP1A1 were identified by GWAS analysis. The association analysis with LEN365 showed the phenotypic variance explained of this haplotype consisting of 5 SNPs is 20.51%. Through transcriptome data analysis, we found the expression of ATP1A1 in the granular layers was increased in the stage of small yellow follicle to large yellow follicle (LYF) and LYF to F5, while decreased in F2 to F1. For the first time, we report the haplotype region formed by 5 SNPS on exon9 of ATP1A1 is associated with egg production in goose and involved in follicle selection and maturation processes.

摘要

鹅肉和鹅蛋是人类食物供应的主要组成部分之一。提高鹅的产蛋量对于不断增长的人口尤为重要。然而，关于鹅产蛋的有效分子标记和机制的信息有限。在这项研究中，我们联合利用不同产蛋四川白鹅基因组重测序和不同卵泡发育阶段转录组的数据，鉴定了产蛋的分子标记和机制。四川白鹅群体个体产蛋量的变异系数为 0.42 至 0.49。将产蛋量最高（产蛋 365 天蛋数 LEN365=79-145）和产蛋量最低（LEN365=8-48）的 50 只个体分为高产蛋量组和低产蛋量组。基于所选样本的全基因组测序数据，鉴定了 36 个与 LEN365 相关的 SNP（注释 novel.12.470、CELF2、ATP1A1、KCNJ6、RAB4A、UST、REV3L、DHX15、CAVN2、SLC5A9、Cldn5、MRPS23 和 Tspan2），涉及代谢和内分泌等多种途径。值得注意的是，通过 GWAS 分析鉴定出 5 个位于 ATP1A1 外显子 9 中的 SNP。与 LEN365 的关联分析表明，由这 5 个 SNP 组成的单倍型的表型方差解释率为 20.51%。通过转录组数据分析，我们发现 ATP1A1 在颗粒层的表达在小卵泡到大黄卵泡（LYF）和 LYF 到 F5 阶段增加，而在 F2 到 F1 阶段减少。我们首次报道，ATP1A1 外显子 9 上由 5 个 SNP 形成的单倍型与鹅的产蛋量有关，参与了卵泡的选择和成熟过程。