一种参与对太平洋牡蛎中CgIFNLP表达进行负调控的IRF2BP成员（CgIRF2BP）。
An IRF2BP member (CgIRF2BP) involved in negative regulation of CgIFNLP expression in oyster Crassostrea gigas.
作者信息
Qiao Xue, Wang Sicong, Zong Yanan, Gu Xiaoyu, Jin Yuhao, Li Youjing, Wei Zhuorui, Wang Lingling, Song Linsheng
机构信息
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, 519000, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
出版信息
Fish Shellfish Immunol. 2023 Mar;134:108576. doi: 10.1016/j.fsi.2023.108576. Epub 2023 Feb 10.
The IRF2BP family of transcription regulators act as corepressor molecules by inhibiting both enhancer-activated and basal transcription involving in many biological contexts. In the present study, an IRF2BP homologue (CgIRF2BP) was identified from oyster C. gigas. Its open reading frame is of 1809 bp encoding a polypeptide of 602 amino acids, which contains an IRF-2BP1_2 domain and a RING domain. The mRNA transcripts of CgIRF2BP were detected in all tested tissues with highest level in haemocytes (28.99-fold of that in mantle, p < 0.05). After poly (I:C) stimulation, the expression level of CgIRF2BP was significantly down-regulated at 3 h (0.50-fold of that in control group, p < 0.001) and gradually increased from 6 h to 48 h (2.69-fold of that in control group, p < 0.01). The recombinant protein of CgIRF2BP (rCgIRF2BP) showed high affinity to both rCgIRF1 and rCgIRF8 with Kd value of 1.02 × 10 and 2.09 × 10, respectively. In CgIRF2BP-RNAi oysters, the mRNA expression of CgIFNLP, CgMx1, CgViperin and CgIFI44L were significantly increased after poly (I:C) stimulation, which were 2.88 (p < 0.01), 1.83 (p < 0.05), 2.47 (p < 0.05), and 1.99-fold (p < 0.01) of that in EGFP group, respectively. These findings suggested that CgIRF2BP negatively regulated CgIFNLP expression by binding with CgIRF1 and CgIRF8.
转录调节因子IRF2BP家族通过抑制增强子激活的转录和基础转录发挥共抑制分子的作用，涉及多种生物学背景。在本研究中，从太平洋牡蛎中鉴定出一个IRF2BP同源物（CgIRF2BP）。其开放阅读框为1809 bp，编码一个602个氨基酸的多肽，包含一个IRF-2BP1_2结构域和一个RING结构域。在所有检测组织中均检测到CgIRF2BP的mRNA转录本，血细胞中的水平最高（是外套膜中的28.99倍，p < 0.05）。经聚（I:C）刺激后，CgIRF2BP的表达水平在3小时时显著下调（为对照组的0.50倍，p < 0.001），并在6小时至48小时逐渐升高（为对照组的2.69倍，p < 0.01）。CgIRF2BP重组蛋白（rCgIRF2BP）对rCgIRF1和rCgIRF8均显示出高亲和力，Kd值分别为1.02×10和2.09×10。在CgIRF2BP-RNA干扰牡蛎中，聚（I:C）刺激后CgIFNLP、CgMx1、CgViperin和CgIFI44L的mRNA表达显著增加，分别是EGFP组的2.88倍（p < 0.01）、1.83倍（p < 0.05）、2.47倍（p < 0.05）和1.99倍（p < 0.01）。这些发现表明，CgIRF2BP通过与CgIRF1和CgIRF8结合负向调节CgIFNLP的表达。
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