State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
Department of Regional Science Operations, La Trobe Rural Health School, Albury-Wodonga, VIC, Australia.
Int J Biol Macromol. 2023 Apr 15;234:123646. doi: 10.1016/j.ijbiomac.2023.123646. Epub 2023 Feb 11.
Lysozyme is used as a food preservative, biological medicine, and infant food additive as a natural anti-infective chemical having bactericidal activity and abundantly secreted in mammals' milk, saliva, etc. We systematically analyzed the 16 coding LYZ genes (C and G-type) in buffalo and cattle to elucidate their evolutionary perspective thoroughly by evaluating an evolutionary relationship, motif patterning, physicochemical attributes, gene, and protein structure, as well as the functional role of the mammary gland-specific expressed buffalo and cattle LYZ genes precisely while considering expression levels difference and the interaction sites variation with bacteria envisaged the potential ability of buffalo LYZ protein with enhanced antibacterial effect. Thus, we speculated that the buffalo mammary glands expressed lysozyme has good antibacterial activity. This study on the buffalo lysozyme gene family not only provides comprehensive insights into the genetic architecture and their antibacterial effect but also offers a theoretical basis for the development of new veterinary drugs and animal health care for mastitis, as well as a new molecular genetic basis to study food or medical lysozyme.
溶菌酶被用作食品防腐剂、生物医学和婴儿食品添加剂，是一种具有杀菌活性的天然抗感染化学物质，在哺乳动物的乳汁、唾液等中大量分泌。我们系统地分析了水牛和牛的 16 个编码 LYZ 基因（C 型和 G 型），通过评估进化关系、基序模式、理化属性、基因和蛋白质结构，以及对乳腺特异性表达的水牛和牛 LYZ 基因的功能作用进行了全面的分析，同时考虑到与细菌的表达水平差异和相互作用位点的变化，预测了水牛 LYZ 蛋白增强抗菌效果的潜力。因此，我们推测水牛乳腺表达的溶菌酶具有良好的抗菌活性。本研究对水牛溶菌酶基因家族不仅提供了对遗传结构及其抗菌作用的全面了解，也为乳腺炎的新型兽药和动物保健的发展提供了理论基础，以及研究食品或医学溶菌酶的新的分子遗传基础。
