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弹涂鱼(大弹涂鱼和青弹涂鱼)中与磷酸烯醇丙酮酸代谢相关的酶的活性

Activities of enzymes associated with phosphoenolpyruvate metabolism in the mudskippers, Boleophthalmus boddaerti and Periophthalmodon schlosseri.

作者信息

Siau H, Ip Y K

机构信息

Department of Zoology, National University of Singapore, Kent Ridge.

出版信息

Comp Biochem Physiol B. 1987;88(1):119-25. doi: 10.1016/0305-0491(87)90089-7.

Abstract
  1. PK and LDH activities in the muscle of Periophthalmodon schlosseri and Boleophthalmus boddaerti were at least 100-fold higher than their respective activities in the liver. 2. The ratio of PK:PEPCK in liver of B. boddaerti was smaller than that of P. schlosseri. 3. PK:PEPCK ratios in both fishes were intermediate between those of aerobic and anaerobic organisms. 4. MDH activity was higher than other enzymes assayed in the liver of both fishes. 5. The ratios of LDH:MDH in the liver of both mudskippers were comparable to those of anaerobic organisms. 6. AST was at least eight times more active than ALT in the liver of both fishes. 7. In the muscle of these mudskippers, the aspartate content was significantly less than that of alanine. 8. Exposure of these fishes to various experimental conditions led to changes in specific activities of PEPCK, LDH, AST and ALT.
摘要
  1. 弹涂鱼和大弹涂鱼肌肉中的磷酸激酶(PK)和乳酸脱氢酶(LDH)活性至少比它们肝脏中的各自活性高100倍。2. 大弹涂鱼肝脏中PK:磷酸烯醇式丙酮酸羧激酶(PEPCK)的比率小于弹涂鱼的该比率。3. 两种鱼类的PK:PEPCK比率介于需氧生物和厌氧生物的该比率之间。4. 苹果酸脱氢酶(MDH)活性高于两种鱼类肝脏中检测的其他酶活性。5. 两种弹涂鱼肝脏中LDH:MDH的比率与厌氧生物的该比率相当。6. 两种鱼类肝脏中天冬氨酸转氨酶(AST)的活性至少比丙氨酸转氨酶(ALT)高八倍。7. 在这些弹涂鱼的肌肉中,天冬氨酸含量明显低于丙氨酸含量。8. 将这些鱼类暴露于各种实验条件下会导致PEPCK、LDH、AST和ALT的比活性发生变化。

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